Document Detail


An oleate hydroxylase from the fungus Claviceps purpurea: cloning, functional analysis, and expression in Arabidopsis.
MedLine Citation:
PMID:  18467452     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Claviceps purpurea, a fungal pathogen responsible for ergot diseases in many agriculturally important cereal crops, produces high levels of ricinoleic acid (12-hydroxyoctadec-cis-9-enoic acid) in its sclerotia. It has been believed for many years that the biosynthesis of this fatty acid in C. purpurea involves a hydration process with linoleic acid as the substrate. Using degenerate polymerase chain reaction, we cloned a gene from the sclerotia encoding an enzyme (CpFAH) that has high sequence similarity to the C. purpurea oleate desaturase, but only low similarity to plant oleate hydroxylases. Functional analysis of CpFAH in yeast (Saccharomyces cerevisiae) indicated it acted predominantly as a hydroxylase, introducing hydroxyl groups at the 12-position of oleic acid and palmitoleic acid. As well, it showed Delta(12) desaturase activities on 16C and 18C monounsaturated fatty acids and, to a much lesser extent, omega(3) desaturase activities on ricinoleic acid. Heterologous expression of CpFAH under the guidance of a seed-specific promoter in Arabidopsis (Arabidopsis thaliana) wild-type and mutant (fad2/fae1) plants resulted in the accumulation of relatively higher levels of hydroxyl fatty acids in seeds. These data indicate that the biosynthesis of ricinoleic acid in C. purpurea is catalyzed by the fungal desaturase-like hydroxylase, and CpFAH, the first Delta(12) oleate hydroxylase of nonplant origin, is a good candidate for the transgenic production of hydroxyl fatty acids in oilseed crops.
Authors:
Dauenpen Meesapyodsuk; Xiao Qiu
Publication Detail:
Type:  Journal Article     Date:  2008-05-08
Journal Detail:
Title:  Plant physiology     Volume:  147     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-09     Completed Date:  2008-09-15     Revised Date:  2010-09-22    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1325-33     Citation Subset:  IM    
Affiliation:
Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5A8.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU661785
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / enzymology,  genetics
Claviceps / enzymology*,  genetics
Cloning, Molecular
Fatty Acid Desaturases / genetics,  metabolism*
Fungal Proteins / genetics,  metabolism*
Gene Expression
Molecular Sequence Data
Oleic Acid / metabolism*
Saccharomyces cerevisiae / enzymology,  genetics
Seeds / metabolism
Chemical
Reg. No./Substance:
0/Fungal Proteins; 112-80-1/Oleic Acid; EC 1.14.19.-/Fatty Acid Desaturases
Comments/Corrections

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