| An oleate hydroxylase from the fungus Claviceps purpurea: cloning, functional analysis, and expression in Arabidopsis. | |
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MedLine Citation:
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PMID: 18467452 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Claviceps purpurea, a fungal pathogen responsible for ergot diseases in many agriculturally important cereal crops, produces high levels of ricinoleic acid (12-hydroxyoctadec-cis-9-enoic acid) in its sclerotia. It has been believed for many years that the biosynthesis of this fatty acid in C. purpurea involves a hydration process with linoleic acid as the substrate. Using degenerate polymerase chain reaction, we cloned a gene from the sclerotia encoding an enzyme (CpFAH) that has high sequence similarity to the C. purpurea oleate desaturase, but only low similarity to plant oleate hydroxylases. Functional analysis of CpFAH in yeast (Saccharomyces cerevisiae) indicated it acted predominantly as a hydroxylase, introducing hydroxyl groups at the 12-position of oleic acid and palmitoleic acid. As well, it showed Delta(12) desaturase activities on 16C and 18C monounsaturated fatty acids and, to a much lesser extent, omega(3) desaturase activities on ricinoleic acid. Heterologous expression of CpFAH under the guidance of a seed-specific promoter in Arabidopsis (Arabidopsis thaliana) wild-type and mutant (fad2/fae1) plants resulted in the accumulation of relatively higher levels of hydroxyl fatty acids in seeds. These data indicate that the biosynthesis of ricinoleic acid in C. purpurea is catalyzed by the fungal desaturase-like hydroxylase, and CpFAH, the first Delta(12) oleate hydroxylase of nonplant origin, is a good candidate for the transgenic production of hydroxyl fatty acids in oilseed crops. |
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Authors:
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Dauenpen Meesapyodsuk; Xiao Qiu |
Publication Detail:
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Type: Journal Article Date: 2008-05-08 |
Journal Detail:
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Title: Plant physiology Volume: 147 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-09 Completed Date: 2008-09-15 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1325-33 Citation Subset: IM |
Affiliation:
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Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5A8. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EU661785 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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enzymology,
genetics Claviceps / enzymology*, genetics Cloning, Molecular Fatty Acid Desaturases / genetics, metabolism* Fungal Proteins / genetics, metabolism* Gene Expression Molecular Sequence Data Oleic Acid / metabolism* Saccharomyces cerevisiae / enzymology, genetics Seeds / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 112-80-1/Oleic Acid; EC 1.14.19.-/Fatty Acid Desaturases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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