Document Detail


The octadecanoid pathway is required for pathogen-induced multi-functional acetyl-CoA carboxylase accumulation in common bean (Phaseolus vulgaris L.).
MedLine Citation:
PMID:  10960731     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A partial cDNA clone corresponding to the multi-functional acetyl-CoA carboxylase (ACCase, EC 6.4.1.2) was isolated using RNA extracted from methyl jasmonate (MeJA)-induced common bean cell cultures. Most of this clone corresponds to the 3' untranslated region and it showed high identity to alfalfa and soybean ACCase sequences. Southern hybridization revealed one copy of this gene in the common bean genome. In addition to being induced by MeJA in cell cultures and leaves, ACCase mRNA accumulated after yeast elicitor or Pseudomonas syringae pv tabaci treatment. Inhibitors of the octadecanoid pathway severely reduced ACCase mRNA and protein accumulation induced by yeast elicitor or P. syringae pv tabaci, indicating that jasmonates or a precursor mediate ACCase induction after pathogen infection. These results provide a role for the eukaryotic ACCase during the defense response to pathogens in common bean.
Authors:
García-Ponce; Rocha-Sosa
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Plant science (Shannon, Ireland)     Volume:  157     ISSN:  0168-9452     ISO Abbreviation:  Plant Sci.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-08-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882015     Medline TA:  Plant Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  181-190     Citation Subset:  -    
Affiliation:
Instituto de Biotecnología, Universidad Nacional Autónoma de México. Apdo. Postal 510-3, Cuernavaca, 62250, Morelos, Mexico
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