| The occurrence of heritable Mu excisions in starving cells of Escherichia coli. | |
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MedLine Citation:
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PMID: 7957089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A strain of Escherichia coli constructed by Shapiro has a segment of Mu bacteriophage DNA inserted between the araC and lacZ genes. Excision events that produce an in-frame fusion of lacZ to araB result in a cell (here designated Ara-Lac+) that can grow on lactose if arabinose is present as an inducer. Whether or not these excision events occur in the absence of selection for the Ara-Lac+ phenotype has figured prominently in the debate of the phenomenon known as 'directed' or 'adaptive' mutation. In an attempt to settle the issue, we have used classic fluctuation tests to show that cells capable of producing a clone of descendants that are phenotypically Ara-Lac+ do, indeed, arise in stationary phase cultures kept starving in depleted minimal medium. We found that Ara-Lac+ progenitors arise rapidly under these conditions, in contrast to the delayed appearance of Ara-Lac+ mutants when cells are incubated on lactose-arabinose minimal plates. Similar results are reported in the accompanying paper by Maenhaut-Michel and Shapiro, who used indirect selection to isolate Ara-Lac+ cells in the absence of selection. However, their sequencing data have introduced a new unexpected complication to the interpretation of all such experiments, and it is no longer clear exactly when the fusions arise. |
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Authors:
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P L Foster; J Cairns |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The EMBO journal Volume: 13 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1994-12-19 Completed Date: 1994-12-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 5240-4 Citation Subset: IM |
Affiliation:
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Department of Environmental Health, Boston University School of Medicine. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabinose
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pharmacology Bacteriophage mu / genetics* Culture Media Enzyme Induction Escherichia coli / genetics*, virology Genes, araC / genetics* Lac Operon / genetics* Lactose / metabolism Mutagenesis Reading Frames / genetics Recombinant Fusion Proteins / genetics* Selection, Genetic Tetracycline Resistance Virus Activation / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Recombinant Fusion Proteins; 147-81-9/Arabinose; 63-42-3/Lactose |
| Comments/Corrections | |
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