Document Detail


The occurrence of gastric and duodenal auto-antibodies in peptic ulcer disease.
MedLine Citation:
PMID:  2532448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The possible relationship between peptic ulcer and the occurrence of auto-antibodies was investigated by means of an enzyme-linked immunosorbent assay (ELISA). Sera from 24 patients with active duodenal ulcer were analysed using cells and subcellular fractions from pig gastric acid duodenal mucosa for binding of immunoglobulins. Four sera (17%) reacted with a homogenate from duodenal mucosa. Nine sera (38%) were found to contain auto-antibodies against gastric mucosal cells. The cell-reactive auto-antibodies were shown to bind preferentially to parietal cells and chief cells. In these cells the antigens were identified as H, K-ATPase and pepsinogen respectively. Six sera were positive against purified H,K-ATPase; 12 sera were positive against pepsinogen, and only one of these sera reacted with both H,K-ATPase and pepsinogen. The results show that auto-antibodies are formed in a large fraction of patients (18/24; 75%) with peptic ulcer disease. The present study further demonstrates that enrichment of antigenic structures is required for obtaining a satisfactory sensitivity in the assay.
Authors:
Y H Song; S Mårdh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  137     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-02-05     Completed Date:  1990-02-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  535-9     Citation Subset:  IM    
Affiliation:
Department of Medical and Physiological Chemistry, Uppsala University, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / immunology
Animals
Autoantibodies / analysis*
Duodenal Ulcer / immunology*
Duodenum / immunology*
Enzyme-Linked Immunosorbent Assay
Gastric Mucosa / immunology*
Humans
Immunoglobulin G / metabolism
Intestinal Mucosa / immunology*
Pepsinogens / immunology
Swine
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Immunoglobulin G; 0/Pepsinogens; EC 3.6.1.-/Adenosine Triphosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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