Document Detail


An observational study of the microbiome of the maternal pouch and saliva of the tammar wallaby, Macropus eugenii, and of the gastrointestinal tract of the pouch young.
MedLine Citation:
PMID:  19833775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Marsupial mammals, born in an extremely atricial state with no functional immune system, offer a unique opportunity to investigate both the developing microbiome and its relationship to that of the mother and the potential influence of this microbiome upon the development of the immune system. In this study we used a well-established marsupial model animal, Macropus eugenii, the tammar wallaby, to document the microbiome of three related sites: the maternal pouch and saliva, and the gastrointestinal tract (GIT) of the young animal. We used molecular-based methods, targeting the 16S rDNA gene to determine the bacterial diversity at these study sites. In the maternal pouch, 41 unique phylotypes, predominantly belonging to the phylum Actinobacteria, were detected, while in the saliva, 48 unique phylotypes were found that predominantly belonged to the phylum Proteobacteria. The GIT of the pouch young had a complex microbiome of 53 unique phylotypes, even though the pouch young were still permanently attached to the teat and had only been exposed to the external environment for a few minutes immediately after birth while making their way from the birth canal to the maternal pouch. Of these 53 phylotypes, only nine were detected at maternal sites. Overall, the majority of bacteria isolated were novel species (<97 % identity to known 16S rDNA sequences), and each study site (i.e. maternal pouch and saliva, and the GIT of the pouch young) possessed its own unique microbiome.
Authors:
Kim-Ly Chhour; Lyn A Hinds; Nicholas A Jacques; Elizabeth M Deane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-15
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  156     ISSN:  1465-2080     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-04     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  798-808     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, NSW 2109, Australia.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/GQ358790;  GQ358791;  GQ358792;  GQ358793;  GQ358794;  GQ358795;  GQ358796;  GQ358797;  GQ358798;  GQ358799;  GQ358800;  GQ358801;  GQ358802;  GQ358803;  GQ358804;  GQ358805;  GQ358806;  GQ358807;  GQ358808;  GQ358809;  GQ358810;  GQ358811;  GQ358812;  GQ358813;  GQ358814;  GQ358815;  GQ358816;  GQ358817;  GQ358818;  GQ358819;  GQ358820;  GQ358821;  GQ358822;  GQ358823;  GQ358824;  GQ358825;  GQ358826;  GQ358827;  GQ358828;  GQ358829;  GQ358830;  GQ358831;  GQ358832;  GQ358833;  GQ358834;  GQ358835;  GQ358836;  GQ358837;  GQ358838;  GQ358839;  GQ358840;  GQ358841;  GQ358842;  GQ358843;  GQ358844;  GQ358845;  GQ358846;  GQ358847;  GQ358848;  GQ358849;  GQ358850;  GQ358851;  GQ358852;  GQ358853;  GQ358854;  GQ358855;  GQ358856;  GQ358857;  GQ358858;  GQ358859;  GQ358860;  GQ358861;  GQ358862;  GQ358863;  GQ358864;  GQ358865;  GQ358866;  GQ358867;  GQ358868;  GQ358869;  GQ358870;  GQ358871;  GQ358872;  GQ358873;  GQ358874;  GQ358875;  GQ358876;  GQ358877;  GQ358878;  GQ358879;  GQ358880;  GQ358881;  GQ358882;  GQ358883;  GQ358884;  GQ358885;  GQ358886;  GQ358887;  GQ358888;  GQ358889;  GQ358890;  GQ358891;  GQ358892;  GQ358893;  GQ358894;  GQ358895;  GQ358896;  GQ358897;  GQ358898;  GQ358899;  GQ358900;  GQ358901;  GQ358902;  GQ358903;  GQ358904;  GQ358905;  GQ358906;  GQ358907;  GQ358908;  GQ358909;  GQ358910;  GQ358911;  GQ358912;  GQ358913;  GQ358914;  GQ358915;  GQ358916;  GQ358917;  GQ358918
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MeSH Terms
Descriptor/Qualifier:
Actinobacteria / isolation & purification
Animals
DNA, Bacterial / genetics
Female
Gastrointestinal Tract / microbiology*
Macropodidae / microbiology*
Metagenome*
Molecular Sequence Data
Phylogeny
Proteobacteria / isolation & purification
RNA, Ribosomal, 16S / genetics
Saliva / microbiology*
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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