Document Detail

A numerical study of cell behaviour in a ternary solution during the freezing process.
MedLine Citation:
PMID:  12908026     Owner:  NLM     Status:  MEDLINE    
Using a continuum model for multi-component phase change system, the freezing of cell suspension in a ternary solution, H2O-NaCl-CPA (cryoprotective agent) inside a flat bag is investigated numerically in this study. The temperature and phase change history, intracellular water loss, and the volume change of the cells at different locations inside cell suspension are calculated. Numerical results reveal that although the sample boundary is cooled at a constant rate, different locations inside the sample experienced different temperature changes and cooling rates. The highest cooling rates occur at internal locations. The cell volume change is location-dependent.
Dawei Luo; Liqun He; Shuxia Cheng; Jianping Yu; Dayong Gao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  24     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2003 May-Jun
Date Detail:
Created Date:  2003-08-08     Completed Date:  2003-11-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  161-70     Citation Subset:  IM    
Department of Mechanical Engineering, University of Kentucky, Lexington, KY 40506, USA.
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MeSH Terms
Cell Physiological Phenomena*
Cell Size
Cryoprotective Agents
Dimethyl Sulfoxide
Models, Biological*
Sodium Chloride
Reg. No./Substance:
0/Cryoprotective Agents; 0/Solutions; 67-68-5/Dimethyl Sulfoxide; 7647-14-5/Sodium Chloride; 7732-18-5/Water

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