Document Detail

A number of subgenomic DNAs are produced following agroinoculation of plants with beet curly top virus.
MedLine Citation:
PMID:  1538189     Owner:  NLM     Status:  MEDLINE    
In addition to ss and ds genomic DNA, agroinoculation of Nicotiana benthamiana plants with the Logan strain of the geminivirus beet curly top virus (BCTV) consistently resulted in de novo production of subgenomic DNAs on initial passage. Single-stranded and dsDNA forms representing at least seven size classes (0.8 to 1.8 kb) of subgenomic DNA were observed in total DNA extracts from inoculated plants. Extracts from infected sugar beet and tomato contained variable but usually smaller amounts of subgenomic DNAs, suggesting that their production may be influenced by the host species. Restriction endonuclease mapping and partial nucleotide sequencing of three independent clones of a 1.5 kb size class indicated that this subgenomic DNA is produced from the standard viral genome by two separate deletion events. One deletion of 941 bp includes portions of the leftward open reading frames (ORFs) L1, L2 and L3, while the other deletion of 579 bp encompasses portions of the intergenic region and the rightward ORFs R1, R2 and R3. The data indicate that the 1.5 kb BCTV subgenomic DNA is a defective DNA that has retained cis-elements essential for replication.
D C Stenger; M C Stevenson; S G Hormuzdi; D M Bisaro
Related Documents :
6092069 - Encephalomyocarditis virus replication complexes preferentially utilizing nucleoside di...
9811749 - Identification and characterization of the escherichia coli-expressed rna-dependent rna...
24891379 - Dna stool test recommended as screening tool.
85719 - In vitro synthesis of infectious transforming dna by the avian sarcoma virus reverse tr...
25063339 - Can the use of medical muds cause genotoxicity in eukaryotic cells? a trial using comet...
21291179 - Mpic: a high-throughput analytical method for multiple dna targets.
23760279 - A conserved motif in tetrahymena thermophila telomerase reverse transcriptase is proxim...
16923339 - Efficient inhibition of human telomerase activity by antisense oligonucleotides sensiti...
7693119 - Flow cytometry and rna studies.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of general virology     Volume:  73 ( Pt 2)     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-03-27     Completed Date:  1992-03-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  237-42     Citation Subset:  IM    
Biotechnology Center, Ohio State University, Columbus 43210.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Base Sequence
Blotting, Southern
DNA Replication*
DNA, Viral / analysis,  biosynthesis*,  chemistry
Molecular Sequence Data
Plant Viruses / genetics*,  physiology
Plants, Toxic*
Restriction Mapping
Tobacco / microbiology*
Virus Replication*
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cooperative binding of the red clover necrotic mosaic virus movement protein to single-stranded nucl...
Next Document:  The myxoma virus thymidine kinase gene: sequence and transcriptional mapping.