Document Detail

A number of subgenomic DNAs are produced following agroinoculation of plants with beet curly top virus.
MedLine Citation:
PMID:  1538189     Owner:  NLM     Status:  MEDLINE    
In addition to ss and ds genomic DNA, agroinoculation of Nicotiana benthamiana plants with the Logan strain of the geminivirus beet curly top virus (BCTV) consistently resulted in de novo production of subgenomic DNAs on initial passage. Single-stranded and dsDNA forms representing at least seven size classes (0.8 to 1.8 kb) of subgenomic DNA were observed in total DNA extracts from inoculated plants. Extracts from infected sugar beet and tomato contained variable but usually smaller amounts of subgenomic DNAs, suggesting that their production may be influenced by the host species. Restriction endonuclease mapping and partial nucleotide sequencing of three independent clones of a 1.5 kb size class indicated that this subgenomic DNA is produced from the standard viral genome by two separate deletion events. One deletion of 941 bp includes portions of the leftward open reading frames (ORFs) L1, L2 and L3, while the other deletion of 579 bp encompasses portions of the intergenic region and the rightward ORFs R1, R2 and R3. The data indicate that the 1.5 kb BCTV subgenomic DNA is a defective DNA that has retained cis-elements essential for replication.
D C Stenger; M C Stevenson; S G Hormuzdi; D M Bisaro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of general virology     Volume:  73 ( Pt 2)     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-03-27     Completed Date:  1992-03-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  237-42     Citation Subset:  IM    
Biotechnology Center, Ohio State University, Columbus 43210.
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MeSH Terms
Base Sequence
Blotting, Southern
DNA Replication*
DNA, Viral / analysis,  biosynthesis*,  chemistry
Molecular Sequence Data
Plant Viruses / genetics*,  physiology
Plants, Toxic*
Restriction Mapping
Tobacco / microbiology*
Virus Replication*
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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