Document Detail

The number of chloride-cation cotransport sites on Ehrlich ascites cells measured with [3H]bumetanide.
MedLine Citation:
PMID:  3706518     Owner:  NLM     Status:  MEDLINE    
Simultaneous measurements were made of net Cl influxes and [3H]bumetanide binding to Ehrlich ascites tumor cells in which the chloride-cation cotransport pathway had been activated by hypertonic challenge. There was a good linear correlation between inhibition of Cl influx during regulatory volume increase and numbers of bumetanide molecules bound per cell, consistent with high specificity of bumetanide binding to cotransport sites. The extrapolation to the number of bumetanide binding sites per cell at maximal inhibition of Cl transport thus gives the number of cotransport sites per cell as 2.0 X 10(6). From this, and the fluxes measured (not necessarily maximum fluxes), the turnover number is calculated at 50 Cl ions per site per second. Unstimulated cells in isotonic medium, with negligible bumetanide-inhibitable fluxes, have the same number of bumetanide binding sites as the activated cells undergoing volume regulation.
E K Hoffmann; M Schiødt; P Dunham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  250     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-06-18     Completed Date:  1986-06-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C688-93     Citation Subset:  IM    
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MeSH Terms
Binding Sites
Biological Transport / drug effects
Bumetanide / metabolism*,  pharmacology
Carcinoma, Ehrlich Tumor / metabolism*
Cell Membrane / metabolism
Chlorides / metabolism*
Diuretics / metabolism*
Grant Support
Reg. No./Substance:
0/Cations; 0/Chlorides; 0/Diuretics; 28395-03-1/Bumetanide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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