| The nucleotide sequence at the transcription termination site of ribosomal RNA in Drosophila melanogaster. | |
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MedLine Citation:
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PMID: 6264393 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The sequence of 300 nucleotides surrounding the transcription termination site for ribosomal RNA in Drosophila melanogaster has been determined. The precise position of the RNA 3'-end was located by sizing rDNA fragments protected from S1 nuclease by hybridization with cytoplasmic 28S rRNA or with nuclear 38S pre-rRNA. Next, the sequence of the first 10 nucleotides at the 3'-end of 28S and 38S rRNA was determined directly. The results indicate the absence of 3'-terminal processing of the 38S pre-rRNA. The nucleotide sequence from a position 27 nucleotides upstream to 150 nucleotides downstream from the termination site was identical in three uninterrupted rRNA genes and one interrupted gene, except for a single base change in the spacer region of one clone. The terminal 100 nucleotides of Drosophila 28S rRNA have more than 60 per cent homology with the corresponding region of Xenopus 28S rRNA and yeast 26S rRNA. |
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Authors:
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R K Mandal; I B Dawid |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleic acids research Volume: 9 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1981 Apr |
Date Detail:
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Created Date: 1981-08-20 Completed Date: 1981-08-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1801-11 Citation Subset: IM |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/K00466; K00467; K00468; V00232 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence DNA / genetics* DNA Restriction Enzymes Drosophila melanogaster / genetics* Molecular Weight RNA, Ribosomal / genetics* Transcription, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Ribosomal; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 24 Month: 4 Year: 1981 Volume: 9 Issue: 8 First Page: 1801 Last Page: 1811 ID: 326804 PubMed Id: 6264393 |
| The nucleotide sequence at the transcription termination site of ribosomal RNA in Drosophila melanogaster. | |
| R K Mandal | |
| I B Dawid | |
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