| The nucleotide pool is a significant target for oxidative stress. | |
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MedLine Citation:
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PMID: 16863995 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidative stress is considered to be one of the most important phenomena involved in the process of aging and age-related diseases. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) has been frequently used as a marker for oxidative stress. However, the origin of extracellular 8-oxo-dG is not well understood. The aim of this work was to investigate the nucleotide pool and the role of the human mutT homologue protein (hMTH1) in the appearance of extracellular 8-oxo-dG in a cellular model system. For this purpose we used primary human fibroblast cells, which were transfected by siRNAs homologous to hMTH1. Extracellular 8-oxo-dG in cell culture media after exposure of the cells to ionizing radiation was measured as enzyme-linked immunosorbent assay reactivity. Our results demonstrate the profound effect of both hMTH1 expression and nucleotide pool size on the cellular excretion of 8-oxo-dG, suggesting that the nucleotide pool is a significant target for the formation of extracellular 8-oxo-dG. |
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Authors:
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Siamak Haghdoost; Lena Sjölander; Stefan Czene; Mats Harms-Ringdahl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-11 |
Journal Detail:
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Title: Free radical biology & medicine Volume: 41 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-07-25 Completed Date: 2006-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
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Languages: eng Pagination: 620-6 Citation Subset: IM |
Affiliation:
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Department of Genetics, Microbiology, and Toxicology, Stockholm University, S 10691 Stockholm, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Cell Line Deoxyguanosine / analogs & derivatives, metabolism Humans Nucleotides / metabolism* Oxidative Stress* RNA, Small Interfering |
| Chemical | |
Reg. No./Substance:
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0/Nucleotides; 0/RNA, Small Interfering; 88847-89-6/8-oxo-7-hydrodeoxyguanosine; 961-07-9/Deoxyguanosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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