| A nucleotide mutation associated with fluoroquinolone resistance observed in gyrA of in vitro obtained Rhodococcus equi mutants. | |
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MedLine Citation:
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PMID: 16563665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, the quinolone resistance-determining region (QRDR) in gyrA and gyrB of in vitro fluoroquinolone-resistant Rhodococcus equi mutants was sequenced. These mutants were selected from four R. equi strains on blood agar plates containing ciprofloxacin or enrofloxacin. Each mutant became 8- to 64 or greater-fold resistant to fluoroquinolones compared with their parent strains. From the results of sequence analysis of QRDR in gyrA and gyrB, a nucleotide mutation of codon GAC for GGC in gyrA was detected in all mutants, but no mutation was observed in gyrB. This mutation leads to amino acid substitution of Asp for Gly in putative GyrA in R. equi. The position of this substitution corresponds to position 87 of GyrA in Escherichia coli. Our results suggest that the mutation of QRDR in gyrA, which was observed in in vitro fluoroquinolone-resistant R. equi mutants in this study, is closely associated with fluoroquinolone resistance. |
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Authors:
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Hidekazu Niwa; Seiji Hobo; Toru Anzai |
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Publication Detail:
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Type: Journal Article Date: 2006-03-23 |
Journal Detail:
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Title: Veterinary microbiology Volume: 115 ISSN: 0378-1135 ISO Abbreviation: Vet. Microbiol. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-29 Completed Date: 2007-08-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705469 Medline TA: Vet Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 264-8 Citation Subset: IM |
Affiliation:
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Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke-shi, Tochigi 329-0412, Japan. niwa@epizoo.equinst.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence DNA Gyrase / chemistry, genetics* Dose-Response Relationship, Drug Drug Resistance, Bacterial / genetics* Fluoroquinolones / pharmacology* Horses Microbial Sensitivity Tests Molecular Sequence Data Mutation* Rhodococcus equi / drug effects*, genetics* |
| Chemical | |
Reg. No./Substance:
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0/Fluoroquinolones; EC 5.99.1.-/DNA Gyrase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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