Document Detail


A nucleosome positioned by alpha2/Mcm1 prevents Hap1 activator binding in vivo.
MedLine Citation:
PMID:  17959145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nucleosome positioning has been proposed as a mechanism of transcriptional repression. Here, we examined whether nucleosome positioning affects activator binding in living yeast cells. We introduced the cognate Hap1 binding site (UAS1) at a location 24-43 bp, 29-48 bp, or 61-80 bp interior to the edge of a nucleosome positioned by alpha2/Mcm1 in yeast minichromosomes. Hap1 binding to the UAS1 was severely inhibited, not only at the pseudo-dyad but also in the peripheral region of the positioned nucleosome in alpha cells, while it was detectable in a cells, in which the nucleosomes were not positioned. Hap1 binding was restored in alpha cells with tup1 or isw2 mutations, which caused the loss of nucleosome positioning. These results support the mechanism in which alpha2/Mcm1-dependent nucleosome positioning has a regulatory function to limit the access of transcription factors.
Authors:
Nobuyuki Morohashi; Kumiko Nakajima; Daichi Kurihara; Yukio Mukai; Aaron P Mitchell; Mitsuhiro Shimizu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-10-16
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  364     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-07     Completed Date:  2007-12-18     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  583-8     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Meisei University, 2-1-1 Hodokubo, Hino, Tokyo 191-8506, Japan.
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MeSH Terms
Descriptor/Qualifier:
DNA-Binding Proteins / genetics*
Nucleosomes / physiology*,  ultrastructure*
Protein Binding
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae Proteins / genetics*
Trans-Activators / genetics*
Transcription Factors / genetics*
Transcriptional Activation / genetics*
Grant Support
ID/Acronym/Agency:
AI070272/AI/NIAID NIH HHS; R01 AI070272-18/AI/NIAID NIH HHS; R01 AI070272-19/AI/NIAID NIH HHS; R01 GM39531/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/HAP1 protein, S cerevisiae; 0/MATA2 protein, S cerevisiae; 0/MCM1 protein, S cerevisiae; 0/Nucleosomes; 0/Saccharomyces cerevisiae Proteins; 0/Trans-Activators; 0/Transcription Factors
Comments/Corrections

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