Document Detail

A nuclear protein tyrosine phosphatase induces shortening of G1 phase and increase in c-Myc protein level.
MedLine Citation:
PMID:  11281638     Owner:  NLM     Status:  MEDLINE    
PTP-S2 is a ubiquitously expressed nuclear protein tyrosine phosphatase which shows increased expression upon mitogenic stimulation in a variety of cells in vitro and in vivo. In order to understand the role of this enzyme in cell cycle progression, tetracycline-regulated HeLa clones expressing PTP-S2 were isolated and characterized. Tetracycline-controlled expression of PTP-S2 increased the rate of cell proliferation. An analysis of the distribution of cells in various phases of the cell cycle in an exponentially growing cell population showed that there was a large decrease in the percentage of cells in G1 phase in a PTP-S2-expressing population of cells compared to nonexpressing cells. This decrease in the percentage of cells in G1 was dependent on the level of PTP-S2 expression. There was a corresponding increase in the percentage of cells in G2/M but no significant increase in the percentage of cells in S phase. An analysis of the time course of cell cycle progression after release from double thymidine block showed that the duration of G1 phase was significantly shortened in cells induced to express exogenous PTP-S2. However, the duration of S phase was not significantly altered and the duration of G2 phase was increased to some extent. Induction of PTP-S2 expression was associated with an increase in c-Myc protein levels, although the c-Myc mRNA level was not changed. Our results suggest that overexpression of PTP-S2 promotes progression of cells through G1 to S phase and is associated with increased level of c-Myc protein through a posttranscriptional mechanism.
U Ganapati; S Gupta; V Radha; C Sudhakar; P S Manogaran; G Swarup
Related Documents :
8516308 - Lethality induced by a single site-specific double-strand break in a dispensable yeast ...
3105868 - Modification of ha-ras oncogene p21 expression and cell cycle progression in the human ...
22309278 - Recombination adenovirus-mediated human lactoferrin cdna inhibits the growth of human m...
11281638 - A nuclear protein tyrosine phosphatase induces shortening of g1 phase and increase in c...
15906158 - In vitro human ependymoblastoma cells differentiate after exposure to nerve growth factor.
21541698 - Searching for "signal 2": costimulation requirements of γδ t cells.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  265     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-03     Completed Date:  2001-05-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Centre for Cellular and Molecular Biology, Hyderabad, 500 007, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle
Cell Division
G1 Phase
Gene Expression
Hela Cells
Nuclear Proteins / biosynthesis*,  genetics
Protein Tyrosine Phosphatases / biosynthesis*,  genetics
Proto-Oncogene Proteins c-myc / genetics,  metabolism*
Tetracycline / pharmacology
Reg. No./Substance:
0/Nuclear Proteins; 0/Proto-Oncogene Proteins c-myc; 60-54-8/Tetracycline; EC Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Neurologic complications after lumbar spine surgery.
Next Document:  Apoptotic cell debris and phosphatidylserine-containing lipid vesicles induce apolipoprotein J (clus...