Document Detail


The nuclear autoimmune antigen Ku is also present on the cell surface.
MedLine Citation:
PMID:  1472635     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyclonal antibodies were raised against the individual 85 and 70 kDa subunits of the Ku complex purified from nuclear extract prepared from the T cell line MLA144. They specifically recognise the appropriate subunits of the Ku complex from whole cell extract of HeLa cells using Western blot analysis. They are also able to identify the Ku proteins present in the cell membrane using FACS analysis.
Authors:
R G Dalziel; S C Mendelson; J P Quinn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Autoimmunity     Volume:  13     ISSN:  0891-6934     ISO Abbreviation:  Autoimmunity     Publication Date:  1992  
Date Detail:
Created Date:  1993-02-04     Completed Date:  1993-02-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8900070     Medline TA:  Autoimmunity     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  265-7     Citation Subset:  IM    
Affiliation:
Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, Summerhall, Edinburgh.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Nuclear*
Autoantigens / analysis*
Cell Membrane / metabolism
Cell Nucleus / metabolism
DNA Helicases*
DNA-Binding Proteins / analysis*
Flow Cytometry
Hela Cells / metabolism
Humans
Nuclear Proteins / analysis*
T-Lymphocytes / metabolism*
Chemical
Reg. No./Substance:
0/Antigens, Nuclear; 0/Autoantigens; 0/DNA-Binding Proteins; 0/Ku autoantigen; 0/Nuclear Proteins; 0/XRCC5 protein, human; EC 3.6.1.-/DNA Helicases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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