| A novel view of albumin-supported sperm capacitation: role of lipid transfer protein-I. | |
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MedLine Citation:
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PMID: 8458468 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine if one mechanism of albumin-mediated support of human sperm capacitation is lipid (cholesterol) transfer activity and contamination of albumin with Lipid Transfer Protein-I (LTP-I). DESIGN AND MAIN OUTCOME MEASURES: Measure lipid transfer activity in various bovine and human albumin preparations, relate this activity to albumin-supported capacitation (measured by zona-free hamster oocyte sperm penetration assay) and acrosome reactions; and attempt to detect LTP-I in active albumins. Remove LTP-I from albumin which supports capacitation and reassess this support. Reconstitute capacitation support by addition of purified LTP-I. SETTING AND SUBJECTS: Healthy sperm donors with normal semen analyses were recruited by the Reproductive Biology-Andrology Laboratory in a university medical center. RESULTS: Albumin preparations that effectively support capacitation have high levels of lipid transfer activity and of LTP-I, a protein responsible for lipid transfer activity. Preparations with lower levels of capacitation support have less lipid transfer activity. Removal of LTP-I from supportive albumin significantly reduces the capacitation support, and this is restored by purified LTP-I. Progesterone concentrations in these preparations are negligible. CONCLUSIONS: The variable abilities of albumin preparations to support in vitro sperm capacitation are largely dependent on the presence of contaminating LTP-I. The cholesterol transfer activity of this protein, which is present in human serum and follicular fluid, may be one mechanism in the process of capacitation. |
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Authors:
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S E Ravnik; J J Albers; C H Muller |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Fertility and sterility Volume: 59 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 1993 Mar |
Date Detail:
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Created Date: 1993-04-23 Completed Date: 1993-04-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 629-38 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Washington, Seattle 98195. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrosome
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physiology Animals Blotting, Western Carrier Proteins / analysis, physiology* Cricetinae Female Male Mesocricetus Serum Albumin / analysis, physiology* Sperm Capacitation* Sperm-Ovum Interactions |
| Grant Support | |
ID/Acronym/Agency:
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HD 12629/HD/NICHD NIH HHS; HL 30086/HL/NHLBI NIH HHS; T32 HD 07183/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Serum Albumin; 0/lipid transfer protein |
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