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A novel and versatile sensing platform based on HRP-mimicking DNAzyme-catalyzed template-guided deposition of polyaniline.
MedLine Citation:
PMID:  23079339     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A novel, simple, and versatile amperometric sensing platform was developed based on HRP-mimicking DNAzyme-catalyzed template-guided deposition of polyaniline (PANI). Pb(2+) was chosen as a model to demonstrate the proof-of-concept of our approach. The Pb(2+) aptamer was first self-assembled on the electrode surface. Upon addition of Pb(2+), Pb(2+) bound to its aptamer to form G-quadruplex (G4). HRP-mimicking DNAzyme with peroxidase catalytic activity was successfully generated after hemin was efficiently intercalated into the G4 structure. Subsequently, HRP-mimicking DNAzyme catalyzed oxidation of aniline to PANI with H(2)O(2) exclusively at the G4 structures, which lead to a readily measurable "turn-on" electrochemical signal. The constructed platform exhibited a good linear response toward Pb(2+) over a wide range of concentration from 1.0×10(-9)M to 1.0×10(-6)M with the detection limit of 5.0×10(-10)M. The high performance DNAzyme-based strategy can be further extended to quantify a wide variety of analytes.
Authors:
Feng Li; Limin Yang; Mingqin Chen; Yi Qian; Bo Tang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-2
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  -     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, People's Republic of China; College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
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