| A novel thermophilic pectate lyase containing two catalytic modules of Clostridium stercorarium. | |
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MedLine Citation:
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PMID: 16306696 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Clostridium stercorarium F-9 pel9A gene encodes a pectate lyase Pel9A consisting of 1,240 amino acids with a molecular weight of 135,171. The mature form of Pel9A is a modular enzyme composed of two family-9 catalytic modules of polysaccharide lyases, CM9-1 and CM9-2, in order from the N terminus. Pel9A showed an overall sequence similarity to the hypothetical pectate lyase PelX of Bacillus halodurans (sequence identity 53%), and CM9-2 showed moderate sequence similarities to some pectate lyases of family 9. Sequence identity between CM9-1 and CM9-2 was 21.3%. The full-length Pel9A lacking the N-terminal signal peptide was expressed, purified, and characterized. The enzyme required Ca(2+) ion for its enzyme activity and showed high activity toward polygalacturonic acid but lower activity toward pectin, indicating that Pel9A is a pectate lyase. Immunological analysis using an antiserum raised against the purified enzyme indicated that Pel9A is constitutively synthesized by C. stercorarium F-9. |
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Authors:
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Si Si Hla; Junji Kurokawa; Suryani; Tetsuya Kimura; Kunio Ohmiya; Kazuo Sakka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 69 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-11-24 Completed Date: 2006-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 2138-45 Citation Subset: IM |
Affiliation:
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Faculty of Bioresources, Mie University, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcium Catalytic Domain Cloning, Molecular Clostridium / enzymology* Kinetics Pectins / metabolism Polysaccharide-Lyases / chemistry*, genetics, metabolism Sequence Homology Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Pectins; 7440-70-2/Calcium; 9000-69-5/pectin; 9046-38-2/polygalacturonic acid; EC 4.2.2.-/Polysaccharide-Lyases; EC 4.2.2.2/pectate lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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