| A novel technique for inducing local inner ear damage. | |
| | |
MedLine Citation:
|
PMID: 12583887 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
With significant development of mouse genomics and the availability of transgenic and knockout mice, the mouse will be the preferred animal model for inner ear research. However, few studies have used mice as experimental animals for examination of hair cell degeneration, because of their relative resistance to ototoxic agents and difficulties in surgical treatment. This study presents a model for induction of apoptotic cell death in sensory epithelia of the mouse inner ear using injection of neomycin into the posterior semicircular canal. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay revealed that local application of neomycin produced sufficient induction of apoptotic cell death in both auditory and vestibular epithelia over a definite time course. Supplementation of the general caspase-inhibitor significantly reduced induction of TUNEL-positive cells, indicating caspase-dependency of apoptotic cell death observed in the present model. In addition, the approach to the posterior semicircular canal was an easy technique, and sham-operation induced no significant induction of TUNEL-positive cells. This model, hence, enables the use of various genetic tools in studies for mechanisms of hair cell apoptosis. |
| | |
Authors:
|
Takayuki Nakagawa; Tae Soo Kim; Norihiko Murai; Tsuyoshi Endo; Fukuichiro Iguchi; Ichiro Tateya; Norio Yamamoto; Yasushi Naito; Juichi Ito |
Related Documents
:
|
17117437 - Loss of fgfr3 leads to excess hair cell development in the mouse organ of corti. 8868027 - Effects of hypertrichotic agents on follicular and nonfollicular cells in vitro. 5910007 - Energy balancing in nitella cells treated with dinitrophenol. 16986327 - Organisation of b-cell receptors on the cell membrane. 292817 - Murine leukemia virus-associated cell surface antigens in rats neonatally infected with... 20535037 - Epstein-barr virus latent infection and baff expression in b cells in the multiple scle... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Hearing research Volume: 176 ISSN: 0378-5955 ISO Abbreviation: Hear. Res. Publication Date: 2003 Feb |
Date Detail:
|
Created Date: 2003-02-13 Completed Date: 2003-08-05 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7900445 Medline TA: Hear Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 122-7 Citation Subset: IM |
Affiliation:
|
Department of Otolaryngology-Head and Neck Surgery, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507 Kyoto, Japan. tnakagawa@ent.kuhp.kyoto-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Chloromethyl Ketones
/
pharmacology Animals Anti-Bacterial Agents / administration & dosage* Apoptosis* Caspases / antagonists & inhibitors, metabolism Cochlea / drug effects, physiopathology Cysteine Proteinase Inhibitors / pharmacology Ear, Inner / drug effects*, physiopathology* Epithelium / drug effects, physiopathology Hair Cells, Auditory, Outer / physiopathology In Situ Nick-End Labeling Injections Mice Mice, Inbred C57BL Neomycin / administration & dosage* Saccule and Utricle / drug effects, physiopathology Semicircular Canals / drug effects*, physiopathology* Time Factors Vestibule, Labyrinth / drug effects, physiopathology |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acid Chloromethyl Ketones; 0/Anti-Bacterial Agents; 0/Cysteine Proteinase Inhibitors; 0/benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; 1404-04-2/Neomycin; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Olivocochlear collaterals evoke excitatory effects in onset neurones of the rat cochlear nucleus.
Next Document: Concurrent measurement of distortion product otoacoustic emissions and auditory steady state evoked ...