Document Detail


A novel technique for in situ aggregation of Gluconobacter oxydans using bio-adhesive magnetic nanoparticles.
MedLine Citation:
PMID:  22729662     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here, we present a novel technique to immobilize magnetic particles onto whole Gluconobacter oxydans in situ via a synthetic adhesive biomimetic material inspired by the protein glues of marine mussels. Our approach involves simple coating of a cell adherent polydopamine film onto magnetic nanoparticles, followed by conjugation of the polydopamine-coated nanoparticles to G. oxydans which resulted in cell aggregation. After optimization, 21.3 mg (wet cell weight) G. oxydans per milligram of nanoparticle was aggregated and separated with a magnet. Importantly, the G. oxydan aggregates showed high specific activity and good reusability. The facile approach offers the potential advantages of low cost, easy cell separation, low diffusion resistance, and high efficiency. Furthermore, the approach is a convenient platform technique for magnetization of cells in situ by direct mixing of nanoparticles with a cell suspension.
Authors:
Kefeng Ni; Huimin Lu; Cunxun Wang; Kvar C L Black; Dongzhi Wei; Yuhong Ren; Phillip B Messersmith
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-12
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  109     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-30     Completed Date:  2013-03-20     Revised Date:  2013-12-05    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2970-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion / drug effects*
Cells, Immobilized / chemistry,  cytology*,  metabolism
Equipment Reuse
Gluconobacter oxydans / chemistry,  cytology*,  drug effects*,  metabolism
Hydrogen-Ion Concentration
Indoles / chemistry,  pharmacology
Magnetite Nanoparticles / chemistry*
Osmolar Concentration
Polymers / chemistry,  pharmacology
Sodium Chloride
Temperature
Grant Support
ID/Acronym/Agency:
F31 DE019750/DE/NIDCR NIH HHS; F31 DE019750/DE/NIDCR NIH HHS; R37 DE014193/DE/NIDCR NIH HHS; R37DE014193/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Indoles; 0/Magnetite Nanoparticles; 0/Polymers; 0/polydopamine; 7647-14-5/Sodium Chloride
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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