| A novel technique for in situ aggregation of Gluconobacter oxydans using bio-adhesive magnetic nanoparticles. | |
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MedLine Citation:
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PMID: 22729662 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Here, we present a novel technique to immobilize magnetic particles onto whole Gluconobacter oxydans in situ via a synthetic adhesive biomimetic material inspired by the protein glues of marine mussels. Our approach involves simple coating of a cell adherent polydopamine film onto magnetic nanoparticles, followed by conjugation of the polydopamine-coated nanoparticles to G. oxydans which resulted in cell aggregation. After optimization, 21.3 mg (wet cell weight) G. oxydans per milligram of nanoparticle was aggregated and separated with a magnet. Importantly, the G. oxydan aggregates showed high specific activity and good reusability. The facile approach offers the potential advantages of low cost, easy cell separation, low diffusion resistance, and high efficiency. Furthermore, the approach is a convenient platform technique for magnetization of cells in situ by direct mixing of nanoparticles with a cell suspension. |
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Authors:
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Kefeng Ni; Huimin Lu; Cunxun Wang; Kvar C L Black; Dongzhi Wei; Yuhong Ren; Phillip B Messersmith |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-07-12 |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 109 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-10-30 Completed Date: 2013-03-20 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 2970-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Adhesion
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drug effects* Cells, Immobilized / chemistry, cytology*, metabolism Equipment Reuse Gluconobacter oxydans / chemistry, cytology*, drug effects*, metabolism Hydrogen-Ion Concentration Indoles / chemistry, pharmacology Magnetite Nanoparticles / chemistry* Osmolar Concentration Polymers / chemistry, pharmacology Sodium Chloride Temperature |
| Grant Support | |
ID/Acronym/Agency:
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F31 DE019750/DE/NIDCR NIH HHS; R37 DE014193/DE/NIDCR NIH HHS; R37DE014193/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Indoles; 0/Magnetite Nanoparticles; 0/Polymers; 0/polydopamine; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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