Document Detail

A novel tandem repeat sequence located on human chromosome 4p: isolation and characterization.
MedLine Citation:
PMID:  9192848     Owner:  NLM     Status:  MEDLINE    
In an effort to analyze the genomic region of the distal half of human chromosome 4p, to where Huntington disease and other diseases have been mapped, we have isolated the cosmid clone (CRS447) that was likely to contain a region with specific repeat sequences. Clone CRS447 was subjected to detailed analysis, including chromosome mapping, restriction mapping, and DNA sequencing. Chromosome mapping by both a human-CHO hybrid cell panel and FISH revealed that CRS447 was predominantly located in the 4p15.1-15.3 region. CRS447 was shown to consist of tandem repeats of 4.7-kb units present on chromosome 4p. A single EcoRI unit was subcloned (pRS447), and the complete sequence was determined as 4752 nucleotides. When pRS447 was used as a probe, the number of copies of this repeat per haploid genome was estimated to be 50-70. Sequence analysis revealed that it contained two internal CA repeats and one putative ORF. Database search established that this sequence was unreported. However, two homologous STS markers were found in the database. We concluded that CRS447/pRS447 is a novel tandem repeat sequence that is mainly specific to human chromosome 4p.
M Kogi; S Fukushige; C Lefevre; S Hadano; J E Ikeda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genomics     Volume:  42     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-09-02     Completed Date:  1997-09-02     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  278-83     Citation Subset:  IM    
Ikeda Genosphere Project, ERATO, JRDC, Tokai University School of Medicine, Kanagawa, Japan.
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MeSH Terms
Base Sequence
CHO Cells
Chromosome Mapping
Chromosomes, Human, Pair 4 / genetics*
Cloning, Molecular
DNA Primers / genetics
Dinucleotide Repeats
Genetic Markers
Huntington Disease / genetics
Hybrid Cells
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Open Reading Frames
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid*
Restriction Mapping
Sequence Tagged Sites
Reg. No./Substance:
0/DNA Primers; 0/Genetic Markers

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