| A novel surface modification using tissue factor reconstituted in phospholipid vesicles for the activation of blood coagulation. | |
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MedLine Citation:
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PMID: 19105905 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a novel method to immobilize recombinant human tissue factor (rhTF) reconstituted in phospholipid vesicles. The rhTF vesicles were immobilized in a multilayer vesicle structure using cholesterol-DNA tethers spontaneously inserted into the lipid membrane. The properties of the rhTF vesicle surface modification were characterized by surface plasmon resonance biosensor technology. As an application of this surface modification, we investigated its use as a blood coagulation activating surface. The coagulation activating capacity of the surface modification was tested by exposure to human whole blood in a flow chamber. No increase in rhTF levels in the blood was found after passage through the flow chamber, indicating that the rhTF surface modification was stable. Thrombin-antithrombin (TAT) and prothrombin fragment (PF) 1 + 2 levels increased after exposure to the surface, and decreased in a concentration-dependent way upon addition of melagatran (a direct thrombin inhibitor), i.e., coagulation activity triggered by rhTF could be suppressed by anticoagulation. The results with this new thrombogenic surface are promising, and will be further developed into a useful tool for coagulation related investigations, e.g., characterization of anticoagulants and biomaterials. |
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Authors:
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Sofia Svedhem; Angelica Wikström; Johanna Deinum; Bengt Kasemo; Kenny Hansson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biomaterials science. Polymer edition Volume: 20 ISSN: 0920-5063 ISO Abbreviation: J Biomater Sci Polym Ed Publication Date: 2009 |
Date Detail:
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Created Date: 2008-12-24 Completed Date: 2009-02-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9007393 Medline TA: J Biomater Sci Polym Ed Country: Netherlands |
Other Details:
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Languages: eng Pagination: 133-40 Citation Subset: IM |
Affiliation:
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Department of Applied Physics, Chalmers University of Technology, SE 412 96 Göteborg, Sweden. sofia.svedhem@chalmers.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biological Assay
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instrumentation,
methods Blood Coagulation / drug effects* Humans Phospholipids / chemistry* Recombinant Proteins / chemistry, genetics, pharmacology Surface Properties Thromboplastin / chemistry*, genetics, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids; 0/Recombinant Proteins; 9035-58-9/Thromboplastin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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