| A novel support for antibody purification: fatty acid attached chitosan beads. | |
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MedLine Citation:
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PMID: 19196498 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Linoleic acid attached chitosan beads [poly(LA-Ch)] (1.25 microm in diameter) are obtained by the formation of amide linkages between linoleic acid and chitosan. Poly(LA-Ch) beads are characterized by FTIR, TEM, and swelling studies. Poly(LA-Ch) beads are used for the purification of immunoglobulin-G (IgG) from human plasma in a batch system. The maximum IgG adsorption is observed at pH 7.0 for HEPES buffer. IgG adsorption onto the plain chitosan beads is found to be negligible. Adsorption values up to 136.7 mg/g from aqueous solutions are obtained by poly(LA-Ch) beads. IgG adsorption saw an increase as a result of increasing temperature. Higher amounts of IgG are adsorbed from human plasma (up to 390 mg/g) with a purity of 92%. The adsorption phenomena appeared to follow a typical Langmuir isotherm. It is observed that IgG could be repeatedly adsorbed and desorbed without significant loss when we take into account the adsorption amount. It is concluded that the poly(LA-Ch) beads allowed one-step purification of IgG from human plasma. |
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Authors:
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Deniz Aktaş Uygun; Murat Uygun; Alev Karagözler; Nevra Oztürk; Sinan Akgöl; Adil Denizli |
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Publication Detail:
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Type: Journal Article Date: 2009-01-09 |
Journal Detail:
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Title: Colloids and surfaces. B, Biointerfaces Volume: 70 ISSN: 1873-4367 ISO Abbreviation: Colloids Surf B Biointerfaces Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-03-23 Completed Date: 2009-08-24 Revised Date: 2009-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9315133 Medline TA: Colloids Surf B Biointerfaces Country: Netherlands |
Other Details:
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Languages: eng Pagination: 266-70 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Adnan Menderes University, Aydin, Turkey. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Animals Antibodies / chemistry* Buffers Cattle Chitosan / chemistry* Electrophoresis, Polyacrylamide Gel Fatty Acids / chemistry* Humans Hydrogen-Ion Concentration Immunoglobulin G / chemistry Linoleic Acid / chemistry Microscopy, Electron, Transmission / methods Rabbits Spectroscopy, Fourier Transform Infrared Temperature |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Buffers; 0/Fatty Acids; 0/Immunoglobulin G; 2197-37-7/Linoleic Acid; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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