Document Detail


A novel support for antibody purification: fatty acid attached chitosan beads.
MedLine Citation:
PMID:  19196498     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Linoleic acid attached chitosan beads [poly(LA-Ch)] (1.25 microm in diameter) are obtained by the formation of amide linkages between linoleic acid and chitosan. Poly(LA-Ch) beads are characterized by FTIR, TEM, and swelling studies. Poly(LA-Ch) beads are used for the purification of immunoglobulin-G (IgG) from human plasma in a batch system. The maximum IgG adsorption is observed at pH 7.0 for HEPES buffer. IgG adsorption onto the plain chitosan beads is found to be negligible. Adsorption values up to 136.7 mg/g from aqueous solutions are obtained by poly(LA-Ch) beads. IgG adsorption saw an increase as a result of increasing temperature. Higher amounts of IgG are adsorbed from human plasma (up to 390 mg/g) with a purity of 92%. The adsorption phenomena appeared to follow a typical Langmuir isotherm. It is observed that IgG could be repeatedly adsorbed and desorbed without significant loss when we take into account the adsorption amount. It is concluded that the poly(LA-Ch) beads allowed one-step purification of IgG from human plasma.
Authors:
Deniz Aktaş Uygun; Murat Uygun; Alev Karagözler; Nevra Oztürk; Sinan Akgöl; Adil Denizli
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Publication Detail:
Type:  Journal Article     Date:  2009-01-09
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  70     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-03-23     Completed Date:  2009-08-24     Revised Date:  2009-10-16    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  266-70     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Adnan Menderes University, Aydin, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Animals
Antibodies / chemistry*
Buffers
Cattle
Chitosan / chemistry*
Electrophoresis, Polyacrylamide Gel
Fatty Acids / chemistry*
Humans
Hydrogen-Ion Concentration
Immunoglobulin G / chemistry
Linoleic Acid / chemistry
Microscopy, Electron, Transmission / methods
Rabbits
Spectroscopy, Fourier Transform Infrared
Temperature
Chemical
Reg. No./Substance:
0/Antibodies; 0/Buffers; 0/Fatty Acids; 0/Immunoglobulin G; 2197-37-7/Linoleic Acid; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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