| A novel sulfated structure in the carbohydrate-protein linkage region isolated from porcine intestinal heparin. | |
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MedLine Citation:
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PMID: 1730699 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A preparation of porcine stage 14 intestinal heparin, which contains Ser as a predominant amino acid, was used for isolation of the carbohydrate-protein linkage region of heparin. Two glycoserines were isolated in a molar ratio of 96:4 after an exhaustive digestion with a mixture of bacterial heparinase and heparitinases. Their structures were determined by composition analysis, heparitinase digestion, co-chromatography with an authentic glycoserine on high performance liquid chromatography, and by 500-MHz one- and two-dimensional 1H NMR spectroscopy. The structure of the major one is delta GlcA beta 1-3Gal beta 1-3Gal beta 1-4Xyl beta 1-O-Ser and that of the minor is delta GlcA beta 1-4GlcNAc(6-O-sulfate) alpha 1-4GlcA beta 1-3Gal beta 1-3Gal beta 1-4Xyl beta 1-O-Ser. The novel 6-O-sulfated GlcNAc residue was demonstrated to occur in the vicinity of the carbohydrate-protein linkage region. The Gal residues were nonsulfated, in contrast to the sulfated Gal structures recently discovered in the carbohydrate-protein linkage region of chondroitin sulfate proteoglycans. The structural features are discussed in relation to biosynthetic mechanisms of the heparin glycosaminoglycans. |
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Authors:
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K Sugahara; S Yamada; K Yoshida; P de Waard; J F Vliegenthart |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 267 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1992 Jan |
Date Detail:
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Created Date: 1992-02-18 Completed Date: 1992-02-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1528-33 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Conformation Carbohydrate Sequence Chromatography, High Pressure Liquid Heparin / chemistry*, isolation & purification Heparin Lyase Heparitin Sulfate / chemistry*, isolation & purification Intestines / chemistry* Magnetic Resonance Spectroscopy Molecular Sequence Data Oligosaccharides / chemistry, isolation & purification Polysaccharide-Lyases Serine / analysis Swine |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides; 56-45-1/Serine; 9005-49-6/Heparin; 9050-30-0/Heparitin Sulfate; EC 4.2.2.-/Polysaccharide-Lyases; EC 4.2.2.7/Heparin Lyase; EC 4.2.2.8/heparitinsulfate lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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