| A novel strategy for preventing PERV transmission to human cells by remodeling the viral envelope glycoprotein. | |
| | |
MedLine Citation:
|
PMID: 16756569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Porcine endogenous retrovirus (PERV) released from pig cells is a main problem associated with clinical xenotransplantation. In a previous study, we demonstrated that the high mannose type of N-glycan of the envelope glycoprotein is closely related to PERV infectivity with respect to human cells. In this study, we addressed the effects of reducing the high mannose type of N-glycan on PERV infectivity. METHODS: Pig endothelial cells (PEC) were transduced with the LacZ gene by a pseudotype infection to produce PEC(Z). The PEC(Z)s were then further infected with PERV subtype B (PERV-B) to produce PEC(Z)/PB. The PEC(Z)/PBs were next transfected with the alpha 1,2 mannosidase Ib (Man Ib), N-acetylglucosaminyltransferase I (GnT-I) or alpha-mannosidase II (Man II) gene in order to reduce the levels of high mannose type of N-glycan. HEK293 cells were inoculated with the PERV in each of the culture supernatants. The inoculated cells were histochemically stained and the LacZ-positive cells were counted. RESULTS: In experiment I, PERV transmission from the PEC(Z)/PB with GnT-I or Man II to HEK 293 cells was significantly reduced in comparison with control PEC(Z)/PB, while the PEC(Z)/PB with Man Ib was not. However, in experiment II, PERV transmission from the PEC(Z)/PB with ManIb to HEK 293 cells was also significantly reduced in comparison with control PEC(Z)/PB. CONCLUSION: The transfection of these genes to pig cells is effective in reducing the susceptibility of human cells to PERV infection. The results suggest that this represents a potentially useful strategy for further decreasing the likelihood of PERV infections. |
| | |
Authors:
|
Shuji Miyagawa; Shino Nakatsu; Kenji Hazama; Takatoshi Nakagawa; Akihiro Kondo; Katsuyoshi Matsunami; Aki Yamamoto; Junko Yamada; Takayuki Miyazawa; Ryota Shirakura |
Related Documents
:
|
19732849 - Improving baculovirus production at high cell density through manipulation of energy me... 12083839 - Cell-associated hiv-1 infection of salivary gland epithelial cell lines. 16547419 - Mosquito cells infected with japanese encephalitis virus release slowly-sedimenting hem... 19120239 - Viral association with the elusive rickettsia of viper plague from ghana, west africa. 403139 - In vitro interaction of neisseria gonorrhoeae type 1 and type 4 with tissue culture cells. 2069719 - High-level expression of a mitochondrial enzyme, ornithine transcarbamylase from rat li... 1481979 - Application of the chemiluminescent assay to cytotoxicity test: detection of menadione-... 23278869 - Development of cranial placodes: insights from studies in chick. 3594729 - Unique carcinogen enhancement of transformation phenotype displayed by cloned rat embry... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Xenotransplantation Volume: 13 ISSN: 0908-665X ISO Abbreviation: Xenotransplantation Publication Date: 2006 May |
Date Detail:
|
Created Date: 2006-06-07 Completed Date: 2006-08-10 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9438793 Medline TA: Xenotransplantation Country: Denmark |
Other Details:
|
Languages: eng Pagination: 258-63 Citation Subset: IM |
Affiliation:
|
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. miyagawa@orgtrp.med.osaka-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Endogenous Retroviruses / genetics, pathogenicity* Humans Kidney Polymerase Chain Reaction Retroviridae Infections / prevention & control* Swine Transduction, Genetic Transplantation, Heterologous* Viral Envelope Proteins / genetics* alpha-Mannosidase / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Viral Envelope Proteins; EC 3.2.1.24/alpha-Mannosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The ethics of xenotransplantation: a survey of student attitudes.
Next Document: Understanding of and attitude to xenotransplantation among Italian university students: impact of a ...