Document Detail

A novel strategy for preventing PERV transmission to human cells by remodeling the viral envelope glycoprotein.
MedLine Citation:
PMID:  16756569     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Porcine endogenous retrovirus (PERV) released from pig cells is a main problem associated with clinical xenotransplantation. In a previous study, we demonstrated that the high mannose type of N-glycan of the envelope glycoprotein is closely related to PERV infectivity with respect to human cells. In this study, we addressed the effects of reducing the high mannose type of N-glycan on PERV infectivity. METHODS: Pig endothelial cells (PEC) were transduced with the LacZ gene by a pseudotype infection to produce PEC(Z). The PEC(Z)s were then further infected with PERV subtype B (PERV-B) to produce PEC(Z)/PB. The PEC(Z)/PBs were next transfected with the alpha 1,2 mannosidase Ib (Man Ib), N-acetylglucosaminyltransferase I (GnT-I) or alpha-mannosidase II (Man II) gene in order to reduce the levels of high mannose type of N-glycan. HEK293 cells were inoculated with the PERV in each of the culture supernatants. The inoculated cells were histochemically stained and the LacZ-positive cells were counted. RESULTS: In experiment I, PERV transmission from the PEC(Z)/PB with GnT-I or Man II to HEK 293 cells was significantly reduced in comparison with control PEC(Z)/PB, while the PEC(Z)/PB with Man Ib was not. However, in experiment II, PERV transmission from the PEC(Z)/PB with ManIb to HEK 293 cells was also significantly reduced in comparison with control PEC(Z)/PB. CONCLUSION: The transfection of these genes to pig cells is effective in reducing the susceptibility of human cells to PERV infection. The results suggest that this represents a potentially useful strategy for further decreasing the likelihood of PERV infections.
Shuji Miyagawa; Shino Nakatsu; Kenji Hazama; Takatoshi Nakagawa; Akihiro Kondo; Katsuyoshi Matsunami; Aki Yamamoto; Junko Yamada; Takayuki Miyazawa; Ryota Shirakura
Related Documents :
11177539 - Manipulation of the cytoplasmic and transmembrane domains alters cell surface levels of...
1064029 - An enzyme system for replication of duplex circular dna: the replicative form of phage ...
16547419 - Mosquito cells infected with japanese encephalitis virus release slowly-sedimenting hem...
19504579 - A flow cytometric assay for the study of e3 ubiquitin ligase activity.
10769059 - Agnoprotein 1a and agnoprotein 1b of avian polyomavirus are apoptotic inducers.
20074329 - Adenovirus-mediated delivery of bfgf small interfering rna increases levels of connexin...
17389399 - Micromechanical control of cell-cell interactions.
19688699 - The role of krüppel-like factors in the reprogramming of somatic cells to induced plur...
22055919 - Comprehensive characterization of two different nicotiana tabacum cell lines leads to d...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Xenotransplantation     Volume:  13     ISSN:  0908-665X     ISO Abbreviation:  Xenotransplantation     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-06-07     Completed Date:  2006-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9438793     Medline TA:  Xenotransplantation     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  258-63     Citation Subset:  IM    
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line
Endogenous Retroviruses / genetics,  pathogenicity*
Polymerase Chain Reaction
Retroviridae Infections / prevention & control*
Transduction, Genetic
Transplantation, Heterologous*
Viral Envelope Proteins / genetics*
alpha-Mannosidase / metabolism
Reg. No./Substance:
0/Viral Envelope Proteins; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The ethics of xenotransplantation: a survey of student attitudes.
Next Document:  Understanding of and attitude to xenotransplantation among Italian university students: impact of a ...