Document Detail


A novel strategy for preventing PERV transmission to human cells by remodeling the viral envelope glycoprotein.
MedLine Citation:
PMID:  16756569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Porcine endogenous retrovirus (PERV) released from pig cells is a main problem associated with clinical xenotransplantation. In a previous study, we demonstrated that the high mannose type of N-glycan of the envelope glycoprotein is closely related to PERV infectivity with respect to human cells. In this study, we addressed the effects of reducing the high mannose type of N-glycan on PERV infectivity. METHODS: Pig endothelial cells (PEC) were transduced with the LacZ gene by a pseudotype infection to produce PEC(Z). The PEC(Z)s were then further infected with PERV subtype B (PERV-B) to produce PEC(Z)/PB. The PEC(Z)/PBs were next transfected with the alpha 1,2 mannosidase Ib (Man Ib), N-acetylglucosaminyltransferase I (GnT-I) or alpha-mannosidase II (Man II) gene in order to reduce the levels of high mannose type of N-glycan. HEK293 cells were inoculated with the PERV in each of the culture supernatants. The inoculated cells were histochemically stained and the LacZ-positive cells were counted. RESULTS: In experiment I, PERV transmission from the PEC(Z)/PB with GnT-I or Man II to HEK 293 cells was significantly reduced in comparison with control PEC(Z)/PB, while the PEC(Z)/PB with Man Ib was not. However, in experiment II, PERV transmission from the PEC(Z)/PB with ManIb to HEK 293 cells was also significantly reduced in comparison with control PEC(Z)/PB. CONCLUSION: The transfection of these genes to pig cells is effective in reducing the susceptibility of human cells to PERV infection. The results suggest that this represents a potentially useful strategy for further decreasing the likelihood of PERV infections.
Authors:
Shuji Miyagawa; Shino Nakatsu; Kenji Hazama; Takatoshi Nakagawa; Akihiro Kondo; Katsuyoshi Matsunami; Aki Yamamoto; Junko Yamada; Takayuki Miyazawa; Ryota Shirakura
Related Documents :
19732849 - Improving baculovirus production at high cell density through manipulation of energy me...
12083839 - Cell-associated hiv-1 infection of salivary gland epithelial cell lines.
16547419 - Mosquito cells infected with japanese encephalitis virus release slowly-sedimenting hem...
19120239 - Viral association with the elusive rickettsia of viper plague from ghana, west africa.
403139 - In vitro interaction of neisseria gonorrhoeae type 1 and type 4 with tissue culture cells.
2069719 - High-level expression of a mitochondrial enzyme, ornithine transcarbamylase from rat li...
1481979 - Application of the chemiluminescent assay to cytotoxicity test: detection of menadione-...
23278869 - Development of cranial placodes: insights from studies in chick.
3594729 - Unique carcinogen enhancement of transformation phenotype displayed by cloned rat embry...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Xenotransplantation     Volume:  13     ISSN:  0908-665X     ISO Abbreviation:  Xenotransplantation     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-06-07     Completed Date:  2006-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9438793     Medline TA:  Xenotransplantation     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  258-63     Citation Subset:  IM    
Affiliation:
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. miyagawa@orgtrp.med.osaka-u.ac.jp
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Endogenous Retroviruses / genetics,  pathogenicity*
Humans
Kidney
Polymerase Chain Reaction
Retroviridae Infections / prevention & control*
Swine
Transduction, Genetic
Transplantation, Heterologous*
Viral Envelope Proteins / genetics*
alpha-Mannosidase / metabolism
Chemical
Reg. No./Substance:
0/Viral Envelope Proteins; EC 3.2.1.24/alpha-Mannosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The ethics of xenotransplantation: a survey of student attitudes.
Next Document:  Understanding of and attitude to xenotransplantation among Italian university students: impact of a ...