Document Detail


A novel strategy of decoy transfection against nuclear factor-kappaB in myocardial preservation.
MedLine Citation:
PMID:  11235718     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Nuclear factor-kappaB (NFkappaB) is critical for the transcription of multiple genes involved in myocardial ischemia-reperfusion injury. Therefore, we hypothesized that blocking NFkappaB would attenuate ischemia-reperfusion injury after prolonged myocardial preservation, resulting in an improvement in cardiac function. METHODS: Double-stranded oligodeoxynucleotides with a specific affinity for NFkappaB (NFkappaB decoy group) or a scrambled decoy group were transfected into rat hearts using a hemagglutinating virus of Japan-liposome method. After 16 hours of preservation in Euro-Collins solution at 4 degrees C, the cardiac grafts were heterotopically transplanted into recipient rats of the same strain. RESULTS: Fluorescein isothiocyanate staining showed introduction of double-stranded oligonucleotides into the nuclei of endothelial cells and cardiomyocytes. After 1 hour of reperfusion the NFkappaB decoy group showed significantly higher degrees of recovery of left ventricular function as well as significantly lower levels of serum creatine phosphokinase, myocardial water content, tissue IL-8, and neutrophil infiltration than did the scrambled decoy group (p < 0.05). CONCLUSIONS: Gene transfection of the NFkappaB decoy attenuates ischemia-reperfusion injury after prolonged heart preservation. As a result, this method appears to be a novel strategy for enhanced myocardial preservation.
Authors:
T Sakaguchi; Y Sawa; N Fukushima; M Nishimura; H Ichikawa; Y Kaneda; H Matsuda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  71     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-03-08     Completed Date:  2001-03-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  624-9; discussion 629-30     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, Course of Interventional Medicine, Osaka University Graduate School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Gene Therapy*
Heart Transplantation / pathology,  physiology
Hemodynamics / physiology
Male
Myocardial Reperfusion Injury / genetics,  pathology,  therapy*
Myocardium / pathology
NF-kappa B / antagonists & inhibitors,  genetics*
Organ Preservation*
Rats
Rats, Sprague-Dawley
Transfection
Treatment Outcome
Ventricular Function, Left / physiology
Chemical
Reg. No./Substance:
0/NF-kappa B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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