Document Detail


A novel stable isotope breath test: 13C-labeled glycosyl ureides used as noninvasive markers of intestinal transit time.
MedLine Citation:
PMID:  7801958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Breath tests are widely used for diagnosis and control of treatment efficacy. There is a need for breath test substrates that reflect intestinal transit times under various conditions. METHODS: We synthesized a new class of breath test substrates, 13C-labeled glycosyl ureides, which are specific markers for the action of colonic microbial flora. We then investigated their usefulness as markers of intestinal transit time. RESULTS: Bolus ingestion of 1 g lactose or cellobiose 13C-ureide resulted in bacterial cleavage of the N-glycosyl bond and subsequent urea hydrolysis. 13CO2 appeared in breath and was determined by gas-isotope-ratio-mass spectrometry. Label first appeared after 5-6 h, peak excretion occurred between 8-14 h, and the signal returned to baseline after 18-24 h. Metoclopramide (10 mg) administered 1 h before the substrate advanced the onset of the signal by 2.4 +/- 1.95 h (mean +/- SD; n = 7; p < 0.05) and the time of peak response by 0.8 +/- 1.44 h. Loperamide (16 mg) broadened the peak and delayed the time of breath signal onset by 1.0 +/- 2.78 h and peak excretion by 4.0 +/- 3.7 h (p < 0.05). CONCLUSIONS: The glycosyl ureide breath test reflected intestinal transit time and rapid changes in gastrointestinal motility. This test should be useful in the diagnosis of a variety of gastrointestinal motility disorders and in the development of drugs that affect gastrointestinal motility.
Authors:
W E Heine; H K Berthold; P D Klein
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The American journal of gastroenterology     Volume:  90     ISSN:  0002-9270     ISO Abbreviation:  Am. J. Gastroenterol.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-01-26     Completed Date:  1995-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421030     Medline TA:  Am J Gastroenterol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  93-8     Citation Subset:  IM    
Affiliation:
USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Breath Tests / methods*
Carbon Dioxide / analysis*
Carbon Radioisotopes / analysis,  diagnostic use*,  urine
Feasibility Studies
Female
Gastrointestinal Transit / drug effects,  physiology*
Glycosylation
Humans
Loperamide / pharmacology
Male
Mass Spectrometry
Metoclopramide / pharmacology
Middle Aged
Urea / analysis*
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 124-38-9/Carbon Dioxide; 364-62-5/Metoclopramide; 53179-11-6/Loperamide; 57-13-6/Urea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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