Document Detail

A novel species of alkaliphilic Bacillus that produces an oxidatively stable alkaline serine protease.
MedLine Citation:
PMID:  11878564     Owner:  NLM     Status:  MEDLINE    
A novel gram-positive, strictly aerobic, motile, sporulating, and facultatively alkaliphilic bacterium designated KSM-KP43 was isolated from a sample of soil. The results of 16S rRNA sequence analysis placed this bacterium in a cluster with Bacillus halmapalus. However, the level of the DNA-DNA hybridization of KSM-KP43 with B. halmapalus was less than 25%. Moreover, the G + C contents of the genomic DNA were 41.6 mol% for KSM-KP43 and 38.6 mol% for B. halmapalus. Because there were also differences in physiological properties and cellular fatty acid composition between the two organisms, we propose KSM-KP43 as a novel species of alkaliphilic Bacillus. This novel strain produces a new class of protease, an oxidatively stable serine protease that is suitable for use in bleach-based detergents. The enzyme contained 640 amino acid residues, including a possible approximately 200-amino-acid prepropeptide in the N-terminal and a unique stretch of approximately 160 amino acids in the C-terminal regions (434-amino-acid mature enzyme with a calculated molecular mass of 45,301 Da). The C-terminal half after the putative catalytic Ser255 and the contiguous C-terminal extension shared local similarity to internal segments of a membrane-associated serine protease of a marine microbial assemblage and the serine protease/ABC transporter precursors of the slime mold Dictyostelium discoideum, and to the C-terminal half of a cold-active alkaline serine protease of a psychrotrophic Shewanella strain.
Katsuhisa Saeki; Jun Hitomi; Mitsuyoshi Okuda; Yuji Hatada; Yasushi Kageyama; Mikio Takaiwa; Hiromi Kubota; Hiroshi Hagihara; Tohru Kobayashi; Shuji Kawai; Susumu Ito
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Extremophiles : life under extreme conditions     Volume:  6     ISSN:  1431-0651     ISO Abbreviation:  Extremophiles     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-03-06     Completed Date:  2002-09-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9706854     Medline TA:  Extremophiles     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  65-72     Citation Subset:  IM; S    
Tochigi Research Laboratories of Kao Corporation, Japan.
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MeSH Terms
Amino Acid Sequence
Bacillus / classification*,  enzymology*,  genetics
Base Sequence
DNA Primers
Hydrogen-Ion Concentration
Molecular Sequence Data
RNA, Ribosomal, 16S / genetics
Sequence Alignment
Sequence Homology, Amino Acid
Serine Endopeptidases / genetics*
Reg. No./Substance:
0/DNA Primers; 0/RNA, Ribosomal, 16S; EC 3.4.21.-/Serine Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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