Document Detail

A novel sodium overload test predicting ascites decompensation in rats with CCl4-induced cirrhosis.
MedLine Citation:
PMID:  15893844     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: We aimed to develop a non-invasive test to identify the initial alterations of sodium homeostasis and prospectively predict decompensation in preascitic cirrhotic rats. METHODS: The sodium overload test (SOT) was performed in control (CT) and CCl4-induced cirrhotic rats (CH) by calculating the percentage of sodium excreted in the urine after NaCl oral administration (0.5 g/kg). Liver fibrosis was quantified by image cytometry. RESULTS: From the 8th week of CCl4 intoxication, while the daily sodium balance did not change in CH and CT, SOT became significantly lower in the former (62.1+/-13.2 vs 78.8+/-13.2%; P=0.035). At sacrifice, ascites was only present in one animal. The degree of liver fibrosis correlated with SOT. In subsequent experiments, 17 cirrhotic rats developed ascites between the 9th and 14th weeks. SOT remained stable up to 3 weeks before ascites appearance, while it fell significantly to 35+/-19 and 26+/-21% at 2 and 1 week before ascites diagnosis, respectively. Nearly all the rats (95%) with a SOT<60% developed ascites within 3 weeks. CONCLUSIONS: In preascitic cirrhotic rats, SOT unveils sodium metabolism abnormalities earlier than the daily sodium balance and prospectively predicts ascites appearance, identifying rats in a homogeneous stage of cirrhosis, which is essential in pathophysiological studies on sodium retention.
Marco Domenicali; Paolo Caraceni; Alessandro Principe; Anna Maria Pertosa; Josefa Ros; Pasquale Chieco; Franco Trevisani; Wladimiro Jiménez; Mauro Bernardi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-04-09
Journal Detail:
Title:  Journal of hepatology     Volume:  43     ISSN:  0168-8278     ISO Abbreviation:  J. Hepatol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-14     Completed Date:  2005-12-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  92-7     Citation Subset:  IM    
Dipartimento di Medicina Interna, Cardioangiologia ed Epatologia, University of Bologna, 40138 Bologna, Italy.
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MeSH Terms
Ascites / etiology*,  physiopathology*
Carbon Tetrachloride
Dose-Response Relationship, Drug
Liver Cirrhosis, Experimental / chemically induced,  complications*,  metabolism*,  pathology
Rats, Wistar
Sodium / metabolism*
Sodium Chloride / administration & dosage,  metabolism,  pharmacology
Survival Analysis
Reg. No./Substance:
56-23-5/Carbon Tetrachloride; 7440-23-5/Sodium; 7647-14-5/Sodium Chloride

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