| A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins. | |
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MedLine Citation:
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PMID: 18059364 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and rapid diagnostics in food microbiology for their unique capability of detecting physiologically hazardous materials. A multi-well plate-based biosensor containing B-cell hybridoma, Ped-2E9, encapsulated in type I collagen matrix, was developed for rapid detection of viable cells of pathogenic Listeria, the toxin listeriolysin O, and the enterotoxin from Bacillus species. This sensor measures the alkaline phosphatase release from infected Ped-2E9 cells colorimetrically. Pathogenic L. monocytogenes cells and toxin preparations from L. monocytogenes or B. cereus showed cytotoxicity ranging from 24 to 98% at 3-6 h postinfection. In contrast, nonpathogenic L. innocua (F4247) and B. subtilis induced minimal cytotoxicity, ranging only 0.4-7.6%. Laser scanning cytometry and cryo-nano scanning electron microscopy confirmed the live or dead status of the infected Ped-2E9 cells in gel matrix. This paper presents the first example of a cell-based sensing system using collagen-encapsulated mammalian cells for rapid detection of pathogenic bacteria or toxin, and demonstrates a potential for onsite use as a portable detection system. |
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Authors:
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Pratik Banerjee; Dominik Lenz; Joseph Paul Robinson; Jenna L Rickus; Arun K Bhunia |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-12-03 |
Journal Detail:
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Title: Laboratory investigation; a journal of technical methods and pathology Volume: 88 ISSN: 1530-0307 ISO Abbreviation: Lab. Invest. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-25 Completed Date: 2008-04-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: United States |
Other Details:
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Languages: eng Pagination: 196-206 Citation Subset: IM |
Affiliation:
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Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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analysis Animals B-Lymphocytes / enzymology* Bacillus / isolation & purification* Bacterial Toxins / analysis*, pharmacology Biological Assay Biosensing Techniques* Cell Death / drug effects Cell Line Cell Membrane / drug effects Cell Survival / drug effects Collagen / pharmacology Laser Scanning Cytometry Listeria / isolation & purification* Mice Microscopy, Electron, Scanning |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Toxins; 9007-34-5/Collagen; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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