Document Detail


A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins.
MedLine Citation:
PMID:  18059364     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and rapid diagnostics in food microbiology for their unique capability of detecting physiologically hazardous materials. A multi-well plate-based biosensor containing B-cell hybridoma, Ped-2E9, encapsulated in type I collagen matrix, was developed for rapid detection of viable cells of pathogenic Listeria, the toxin listeriolysin O, and the enterotoxin from Bacillus species. This sensor measures the alkaline phosphatase release from infected Ped-2E9 cells colorimetrically. Pathogenic L. monocytogenes cells and toxin preparations from L. monocytogenes or B. cereus showed cytotoxicity ranging from 24 to 98% at 3-6 h postinfection. In contrast, nonpathogenic L. innocua (F4247) and B. subtilis induced minimal cytotoxicity, ranging only 0.4-7.6%. Laser scanning cytometry and cryo-nano scanning electron microscopy confirmed the live or dead status of the infected Ped-2E9 cells in gel matrix. This paper presents the first example of a cell-based sensing system using collagen-encapsulated mammalian cells for rapid detection of pathogenic bacteria or toxin, and demonstrates a potential for onsite use as a portable detection system.
Authors:
Pratik Banerjee; Dominik Lenz; Joseph Paul Robinson; Jenna L Rickus; Arun K Bhunia
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-12-03
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  88     ISSN:  1530-0307     ISO Abbreviation:  Lab. Invest.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-25     Completed Date:  2008-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  196-206     Citation Subset:  IM    
Affiliation:
Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / analysis
Animals
B-Lymphocytes / enzymology*
Bacillus / isolation & purification*
Bacterial Toxins / analysis*,  pharmacology
Biological Assay
Biosensing Techniques*
Cell Death / drug effects
Cell Line
Cell Membrane / drug effects
Cell Survival / drug effects
Collagen / pharmacology
Laser Scanning Cytometry
Listeria / isolation & purification*
Mice
Microscopy, Electron, Scanning
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 9007-34-5/Collagen; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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