Document Detail

A novel and sensitive method for the detection of enrofloxacin in food using time-resolved fluoroimmunoassay.
MedLine Citation:
PMID:  23256454     Owner:  NLM     Status:  Publisher    
Abstract A time-resolved fluoroimmunoassay (TRFIA) technique is developed to detect enrofloxacin (ENR) contamination in food. By using ENR-ovalbumin, anti-ENR antibodies, and europium-labeled goat anti-rabbit antibodies, we establish an indirect and competitive method for ENR-TRFIA. The sensitivity of the method is high, with a detection limit of 0.01 ng/mL. The tests show that the technique's sensitivity is 1 μg/kg in eel, pork, and chicken, and 1 μg/L in honey. The detection range attained is 0.01-100 ng/mL and within the detection range the intra- and inter-batch coefficients of variation of the ENR-TRFIA method are 2.4% and 9.2%, respectively. The data obtained from eel samples by TRFIA and enzyme-linked immunoassay are in good agreement. The assay did not cross-react with other quinolones which commonly exist in food. The study suggests that ENR-TRFIA is a simple, sensitive and economic method of screening large quantities of samples, and has good prospects for application.
Zhou Bin; Zhang Kai; Zhang Jue; Wang Ke; Zhao Lili; Jin Jian; Huang Biao
Related Documents :
25029404 - Aflatoxin m1 in raw and imported powdered milk sold in khartoum state, sudan.
24140324 - Nutritional and sensory characteristics of minas fresh cheese made with either goat mil...
24886414 - Food price policies improve diet quality while increasing socioeconomic inequalities in...
21421254 - Mercury in the biotic compartments of northwest patagonia lakes, argentina.
17824434 - Prey density and the behavioral flexibility of a marine predator: the common murre (uri...
2326344 - Histamine in brain may have no role for histaminergic control of food-related drinking ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-20
Journal Detail:
Title:  Toxicology mechanisms and methods     Volume:  -     ISSN:  1537-6524     ISO Abbreviation:  Toxicol. Mech. Methods     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134521     Medline TA:  Toxicol Mech Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, JIangsu, 214063, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Immunotoxicological impact of engineered nanomaterial exposure: mechanisms of immune cell modulation...
Next Document:  The NAD ratio redox paradox: Why does too much reductive power cause oxidative stress?