Document Detail


A novel and sensitive method for the detection of enrofloxacin in food using time-resolved fluoroimmunoassay.
MedLine Citation:
PMID:  23256454     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract A time-resolved fluoroimmunoassay (TRFIA) technique is developed to detect enrofloxacin (ENR) contamination in food. By using ENR-ovalbumin, anti-ENR antibodies, and europium-labeled goat anti-rabbit antibodies, we establish an indirect and competitive method for ENR-TRFIA. The sensitivity of the method is high, with a detection limit of 0.01 ng/mL. The tests show that the technique's sensitivity is 1 μg/kg in eel, pork, and chicken, and 1 μg/L in honey. The detection range attained is 0.01-100 ng/mL and within the detection range the intra- and inter-batch coefficients of variation of the ENR-TRFIA method are 2.4% and 9.2%, respectively. The data obtained from eel samples by TRFIA and enzyme-linked immunoassay are in good agreement. The assay did not cross-react with other quinolones which commonly exist in food. The study suggests that ENR-TRFIA is a simple, sensitive and economic method of screening large quantities of samples, and has good prospects for application.
Authors:
Zhou Bin; Zhang Kai; Zhang Jue; Wang Ke; Zhao Lili; Jin Jian; Huang Biao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-20
Journal Detail:
Title:  Toxicology mechanisms and methods     Volume:  -     ISSN:  1537-6524     ISO Abbreviation:  Toxicol. Mech. Methods     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134521     Medline TA:  Toxicol Mech Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, JIangsu, 214063, PR China.
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