Document Detail

Detoxification of 7-dehydrocholesterol fatal to Helicobacter pylori is a novel role of cholesterol glucosylation.
MedLine Citation:
PMID:  23144252     Owner:  NLM     Status:  MEDLINE    
The glucosylation of free cholesterol (FC) by Helicobacter pylori cells has various biological significances for the survival of this bacterium. H. pylori cells with glucosylated FC are capable of evading host immune systems, such as phagocytosis by macrophages and activation of antigen-specific T cells, and surviving in the gastric mucosal tissues for long periods. An additional role of cholesterol glucosylation in the survival of H. pylori which is distinct from the role of escaping the host immune system, however, has yet to be identified. This study demonstrated that 7-dehydrocholesterol (7dFC), an FC precursor, is a toxic compound fatal to H. pylori cells, but the cell membrane of H. pylori is capable of absorbing this toxic sterol via glucosylation. In contrast to the case with 7dFC, no toxicity to H. pylori cells was detected from the glucosylated 7dFC. In addition, cgt gene mutant H. pylori cells that cannot glucosylate cholesterols had higher susceptibility to the toxic action of 7dFC than wild-type H. pylori cells. These results indicate that the cgt gene product of H. pylori serves to detoxify the sterol fatal to this bacterium and to permit this toxic sterol as a cell membrane lipid component. In summary, this study defined a novel role of cholesterol glucosylation in H. pylori.
Hirofumi Shimomura; Kouichi Hosoda; David J McGee; Shunji Hayashi; Kenji Yokota; Yoshikazu Hirai
Publication Detail:
Type:  Journal Article     Date:  2012-11-09
Journal Detail:
Title:  Journal of bacteriology     Volume:  195     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-27     Completed Date:  2013-02-25     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  359-67     Citation Subset:  IM    
Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, Tochigi, Japan.
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MeSH Terms
Cell Membrane / metabolism
Dehydrocholesterols / metabolism*,  toxicity*
Gene Deletion
Helicobacter pylori / drug effects*,  metabolism*
Microbial Viability / drug effects
Reg. No./Substance:
0/Dehydrocholesterols; BK1IU07GKF/7-dehydrocholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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