Document Detail


A novel repressor-type homeobox gene, ved, is involved in dharma/bozozok-mediated dorsal organizer formation in zebrafish.
MedLine Citation:
PMID:  12351176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dharma/Bozozok (Dha/Boz) is a homeodomain protein containing an Engrailed homology (Eh) 1 repressor motif. It is important in zebrafish dorsal organizer formation. Dha/Boz interacted with a co-repressor Groucho through the Eh1 motif. Expression of a Dha/Boz fused to the transcriptional activator VP16 repressed dorsal axis formation and the expression of organizer genes but led to the dorsal expansion of expression of the homeobox gene vox/vega1, indicating that Dha/Boz functions as a transcriptional repressor for dorsal axis formation. We also isolated a novel homeobox gene, ved, whose expression was negatively regulated by dha/boz. ved's sequence and expression profile were similar to those of vox/vega1 and vent/vega2. Like Vox/Vega1 and Vent/Vega2, Ved acted as a transcriptional repressor. The combined inhibition of ved, vox/vega1, and vent/vega2, by antisense morpholino injection, strongly dorsalized the embryos and elicited ventral expansion of organizer gene expression, compared with the effect of inhibiting each of these genes alone. These results suggest that ved is a target for the repressor Dha/Boz. Ved functions redundantly with vox/vega1 and vent/vega2 to restrict the organizer domain.
Authors:
Takashi Shimizu; Yojiro Yamanaka; Hideaki Nojima; Taijiro Yabe; Masahiko Hibi; Toshio Hirano
Related Documents :
25080366 - Exchange and complementation of genes coding for photosynthetic reaction center core su...
17215296 - The zebrafish zic2a-zic5 gene pair acts downstream of canonical wnt signaling to contro...
7555726 - Goosecoid is not an essential component of the mouse gastrula organizer but is required...
9025066 - The ectodermal control in chick limb development: wnt-7a, shh, bmp-2 and bmp-4 expressi...
20133756 - Reengineering orthogonally selective riboswitches.
17485846 - Polychlorinated biphenyl/biphenyl degrading gene clusters in rhodococcus sp. k37, ha99,...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mechanisms of development     Volume:  118     ISSN:  0925-4773     ISO Abbreviation:  Mech. Dev.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-09-27     Completed Date:  2003-05-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9101218     Medline TA:  Mech Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  125-38     Citation Subset:  IM    
Affiliation:
Department of Molecular Oncology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Motifs
Amino Acid Sequence*
Animals
Blotting, Western
Cell Line
Gene Expression Regulation, Developmental*
Herpes Simplex Virus Protein Vmw65 / metabolism
Homeodomain Proteins / genetics*,  physiology
Humans
In Situ Hybridization
Molecular Sequence Data
Phylogeny
Plasmids / metabolism
Precipitin Tests
Protein Structure, Tertiary
RNA / metabolism
Repressor Proteins / biosynthesis,  genetics*,  physiology*
Sequence Homology, Amino Acid
Transcription, Genetic
Transcriptional Activation
Zebrafish
Zebrafish Proteins / biosynthesis,  genetics*,  physiology*
Chemical
Reg. No./Substance:
0/Herpes Simplex Virus Protein Vmw65; 0/Homeodomain Proteins; 0/Repressor Proteins; 0/Ved protein, zebrafish; 0/Vox protein, zebrafish; 0/Zebrafish Proteins; 0/dharma protein, zebrafish; 0/vent protein, zebrafish; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Programmed cell death in the ascidian embryo: modulation by FoxA5 and Manx and roles in the evolutio...
Next Document:  Plumage pigmentation and expression of its regulatory genes during quail development--histochemical ...