Document Detail


A novel regulatory system required for pathogenicity of Xanthomonas campestris is mediated by a small diffusible signal molecule.
MedLine Citation:
PMID:  9179849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mutations in the seven clustered rpf genes cause downregulated synthesis of extracellular enzymes and reduced virulence of Xanthomonas campestris pathovar campestris (Xcc). The phenotype of mutants in one of the genes, rpfF, can be restored by a diffusible extracellular factor (DSF) produced by all Xcc strains tested, apart from rpfF and rpfB mutants. DSF accumulates in early stationary phase (when synthesis of enzymes is maximal), but levels decline subsequently. Addition of DSF to exponentially-growing wild-type bacteria does not cause precocious enzyme synthesis. rpfB and rpfF are expressed throughout growth, but the rate increases in early stationary phase. RpfB is predicted to be a long-chain fatty acyl CoA ligase, and RpfF shows some relatedness to enoyl CoA hydratases. The properties of DSF suggest that it may be a fatty-acid derivative, and certain lipid preparations possess DSF activity at higher concentrations. These include lipid extracts and acid-hydrolysed lipoplysaccharide and lipid A from Xcc, and purified dodecanoic and hydroxydodecanoic acid. DSF production is confined to certain xanthomonads. We propose a model for the DSF system, which represents a novel mechanism for regulating virulence factor synthesis in response to physiological or environmental changes.
Authors:
C E Barber; J L Tang; J X Feng; M Q Pan; T J Wilson; H Slater; J M Dow; P Williams; M J Daniels
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  24     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-08-13     Completed Date:  1997-08-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  555-66     Citation Subset:  IM    
Affiliation:
Sainsbury Laboratory, John Innes Centre, Norwich Research Park, UK.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Y09700;  Y09701
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MeSH Terms
Descriptor/Qualifier:
Diffusion
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Developmental
Genes, Bacterial
Kinetics
Models, Biological
Multigene Family
Mutation
Phenotype
Signal Transduction
Virulence / genetics
Xanthomonas campestris / genetics,  metabolism,  pathogenicity*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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