Document Detail


A novel recombinator in yeast based on gene II protein from bacteriophage f1.
MedLine Citation:
PMID:  2016047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interchromosomal mitotic recombination in yeast can be stimulated by the protein encoded by gene II of bacteriophage f1. The normal role of the gene II enzyme is to make a site-specific cleavage of a particular strand of the duplex form of the bacteriophage DNA at the origin of DNA replication. The gene II protein was expressed in yeast in an attempt to determine the role of nicked DNA in the initiation of recombination. Stimulation of recombination in yeast by the gene II protein was dependent on the presence of a recognition site for gene II enzyme in the region being assayed. Recombination was stimulated in both directions from the gene II recognition site but showed a directional bias. The distribution of alleles among the recombinants indicated that the chromosome with the gene II recognition site acted as the recipient in gene conversion events.
Authors:
J N Strathern; K G Weinstock; D R Higgins; C B McGill
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  127     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-05-17     Completed Date:  1991-05-17     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  61-73     Citation Subset:  IM    
Affiliation:
Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, Basic Research Program, Maryland 21702-1201.
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MeSH Terms
Descriptor/Qualifier:
Bacteriophages / genetics*
Base Sequence
Binding Sites
Cloning, Molecular
DNA, Fungal / genetics*,  metabolism
Genes, Viral
Ligases / metabolism
Mitosis
Molecular Sequence Data
Recombination, Genetic / genetics*
Saccharomyces cerevisiae / genetics*
Transformation, Genetic
Viral Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
N01-CO-74101/CO/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA replication complex protein, Bacteriophage lambda; 0/DNA, Fungal; 0/Viral Proteins; 0/gene II protein, Enterobacteria phage f1; EC 6.-/Ligases
Comments/Corrections

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