Document Detail


A novel real-time polymerase chain reaction-based method for the detection and quantification of lactose-fermenting Enterobacteriaceae in the dairy and other food industries.
MedLine Citation:
PMID:  20172205     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of lactose-fermenting Enterobacteriaceae and coliforms is routinely assessed to determine the hygienic quality of water and foods, particularly dairy products. This paper reports the use of lacZ-specific primers in an SYBR green I-based real-time PCR method for the easy and rapid detection of coliforms in dairy products. A large number of bacterial species were assayed to establish the specificity of the method. The sensitivity of the method was assessed using artificially contaminated cheeses. The limit of detection was 1 coliform cell in cheese samples enriched for 8h in a culture medium. The entire procedure, including sample processing, enrichment, DNA extraction, and real-time PCR amplification, can be completed within 10 to 12h, making it a single-day assay.
Authors:
M C Mart?n; N Mart?nez; B del Rio; V Ladero; M Fern?ndez; M A Alvarez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  93     ISSN:  1525-3198     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-04-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  860-7     Citation Subset:  IM    
Affiliation:
Instituto de Productos L?cteos de Asturias, CSIC, Asturias, Spain.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/FN297863;  FN297864;  FN297865;  FN297866;  FN297867;  FN297868;  FN297869;  FN297870;  FN297871;  FN297872;  FN297873
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Dairying / methods*
Enterobacteriaceae / genetics,  isolation & purification*
Fermentation
Food Industry / methods*
Lac Operon / genetics
Lactose / metabolism
Molecular Sequence Data
Polymerase Chain Reaction*
Sensitivity and Specificity
Sequence Alignment
Chemical
Reg. No./Substance:
63-42-3/Lactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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