| A novel real-time polymerase chain reaction-based method for the detection and quantification of lactose-fermenting Enterobacteriaceae in the dairy and other food industries. | |
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MedLine Citation:
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PMID: 20172205 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The presence of lactose-fermenting Enterobacteriaceae and coliforms is routinely assessed to determine the hygienic quality of water and foods, particularly dairy products. This paper reports the use of lacZ-specific primers in an SYBR green I-based real-time PCR method for the easy and rapid detection of coliforms in dairy products. A large number of bacterial species were assayed to establish the specificity of the method. The sensitivity of the method was assessed using artificially contaminated cheeses. The limit of detection was 1 coliform cell in cheese samples enriched for 8h in a culture medium. The entire procedure, including sample processing, enrichment, DNA extraction, and real-time PCR amplification, can be completed within 10 to 12h, making it a single-day assay. |
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Authors:
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M C Mart?n; N Mart?nez; B del Rio; V Ladero; M Fern?ndez; M A Alvarez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of dairy science Volume: 93 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-04-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 860-7 Citation Subset: IM |
Affiliation:
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Instituto de Productos L?cteos de Asturias, CSIC, Asturias, Spain. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/FN297863; FN297864; FN297865; FN297866; FN297867; FN297868; FN297869; FN297870; FN297871; FN297872; FN297873 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Dairying / methods* Enterobacteriaceae / genetics, isolation & purification* Fermentation Food Industry / methods* Lac Operon / genetics Lactose / metabolism Molecular Sequence Data Polymerase Chain Reaction* Sensitivity and Specificity Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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63-42-3/Lactose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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