| A novel rat model of abdominal aortic aneurysm using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure. | |
| | |
MedLine Citation:
|
PMID: 19958989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: An ideal animal model of abdominal aortic aneurysm (AAA) is of great importance for clarifying unknown complex mechanisms of the pathogenesis. We introduce a new, simple technique to create reliable AAAs that simulate human aneurysms. METHODS: Experimental models of AAAs were created in 71 rats by means of a 20-minute application of intraluminal elastase (30 U) and extraluminal calcium chloride (0.5M) in the 1-cm segment of infrarenal abdominal aorta (group EC, n = 26). A single application of elastase (group E, n = 24) or calcium chloride (group C, n = 21) was used as control. The treated aorta in each group was measured under physiologic conditions and harvested at 1 and 4 weeks. Successful AAA formation was defined as a dilation ratio >50%. Inflammatory response, elastolytic activity, and histology in the treated aorta were evaluated among the three groups. RESULTS: The surgical procedure in each group was similarly completed for approximate 30 minutes and performed without any technical failure or operative death. At 4 weeks, the dilation ratio and wall thickness were 94.8% +/- 9.9% and 125.4 +/- 5.6 microm in group EC, 43.3% +/- 6.3% and 149.6 +/- 6.5 microm in group E, and 10.9% +/- 4.2% and 152.9 +/- 7.2 microm in group C. The success rate of AAA formation in group EC (92.7%) was significantly higher than that in group E (25.0%) and group C (0.0%). Less elastin content in the aortic wall was observed in group EC. At 1 week, tumor necrosis factor-alpha and interleukin-1beta messenger RNA (mRNA) expressions were significantly upregulated, and CD3+ and CD11b+ cells were significantly infiltrated into the treated aorta of group EC, compared with groups E or C. Gelatinolytic activities and mRNA expressions of matrix metalloproteinase (MMP)-2 and MMP-9 were also significantly activated in group EC. CONCLUSION: The rat AAA model using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure is simple and easy to perform and is highly reliable and reproducible to create a saccular aneurysm similar to human AAAs. This model could be more useful to clarify AAA pathogenesis, mechanisms, and treatment interventions in experimental researches. |
| | |
Authors:
|
Akiko Tanaka; Tomomi Hasegawa; Zhi Chen; Yutaka Okita; Kenji Okada |
Related Documents
:
|
17119979 - Pediatric intracranial aneurysms-clinical characteristics and outcome of surgical treat... 10234999 - The effect and management of delayed vasospasm after aneurysmal subarachnoid hemorrhage. 19043389 - Efficacy of perfusion cooling of the epidural space and cerebrospinal fluid drainage du... 18209619 - Aneurysmal bone cyst recurrence in children: a review of 56 patients. 17382049 - Emergency endovascular stent grafting for thoracic aortic pathology. 12223009 - Long-term results of endovascular aaa repair using a homemade aortomonoiliac ptfe device. 20684929 - Efficacy of median nerve recurrent branch neurectomy as an adjunct to ulnar motor nerve... 21441419 - Outcomes of revision arthroscopic type ii superior labral anterior posterior repairs. 8728219 - A prospective randomized comparison of the swan neck, coiled, and straight tenckhoff ca... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of vascular surgery Volume: 50 ISSN: 1097-6809 ISO Abbreviation: J. Vasc. Surg. Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2009-12-04 Completed Date: 2009-12-21 Revised Date: 2012-10-03 |
Medline Journal Info:
|
Nlm Unique ID: 8407742 Medline TA: J Vasc Surg Country: United States |
Other Details:
|
Languages: eng Pagination: 1423-32 Citation Subset: IM |
Affiliation:
|
Department of Surgery, Division of Cardiovascular Surgery, Kobe University Graduate School of Medicine, Kobe, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD11b / metabolism Antigens, CD3 / metabolism Aorta, Abdominal / drug effects*, metabolism, pathology, surgery Aortic Aneurysm, Abdominal / chemically induced*, metabolism, pathology Calcium Chloride / administration & dosage* Disease Models, Animal* Inflammation / chemically induced Infusions, Intra-Arterial Interleukin-1beta / genetics Male Matrix Metalloproteinase 2 / genetics, metabolism Matrix Metalloproteinase 9 / genetics, metabolism Pancreatic Elastase / administration & dosage* RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Reproducibility of Results Time Factors Tumor Necrosis Factor-alpha / genetics |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD11b; 0/Antigens, CD3; 0/Interleukin-1beta; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 10043-52-4/Calcium Chloride; EC 3.4.21.36/Pancreatic Elastase; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.24/Mmp2 protein, rat; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Impact of peripheral arterial disease on health status: a comparison with chronic heart failure.
Next Document: DNA-array of gene variants in venous leg ulcers: detection of prognostic indicators.