Document Detail

A novel rat 523 amino acid phosphophoryn: nucleotide sequence and genomic organization.
MedLine Citation:
PMID:  11566357     Owner:  NLM     Status:  MEDLINE    
Phosphophoryns (PP), the major noncollagenous proteins (NCPs) in dentin, are believed to play a crucial role in mineral nucleation and hydroxyapatite growth during dentin mineralization. Previously we identified two mature rat PP transcripts, one coding for a 240 amino acid protein (designated as PP(240)) (H.H. Ritchie, L.-H. Wang, J. Biol. Chem. 271 (1996) 21695-21698), and another coding for a 171 amino acid protein (PP(171)) (H. Ritchie, L. Wang, Biochim. Biophys. Acta 1493 (2000) 27-32). We now have identified a third novel dentin sialoprotein (DSP)-PP cDNA transcript that encodes a 523 amino acid protein (PP(523)) with typical PP characteristics including DSS and DS motifs suitable as potential casein kinase I and II phosphorylation sites. Based on amino acid composition, the PP(523) protein product is identical to native rat HP2. We also show that the PP(523) sequence is identical to the corresponding genomic DNA sequence. Taken together, the existence of multiple DSP-PP transcripts, each significantly different from the other in net negative charge, suggests that dentin mineralization processes may be under fine-tune control by these PP protein isoforms.
H H Ritchie; L H Wang; K Knudtson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1520     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-21     Completed Date:  2001-11-01     Revised Date:  2010-02-05    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  212-22     Citation Subset:  IM    
Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, USA.
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MeSH Terms
Amino Acid Sequence
Base Sequence
DNA, Complementary / chemistry
Incisor / metabolism*
Molecular Sequence Data
Phosphoproteins / chemistry,  genetics*
Protein Isoforms / chemistry,  genetics
Protein Precursors / genetics
Sialoglycoproteins / chemistry,  genetics*
Grant Support
Reg. No./Substance:
0/DNA, Complementary; 0/Phosphoproteins; 0/Protein Isoforms; 0/Protein Precursors; 0/Sialoglycoproteins; 0/dentin sialophosphoprotein; 0/phosphophoryn

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