Document Detail


A novel radioimmunoassay of 7-oxo-DHEA and its physiological levels.
MedLine Citation:
PMID:  17298836     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel radioimmunoassay (RIA) of unconjugated 7-oxo-dehydroepiandrosterone (7-oxo-DHEA) in human serum was developed for the first time. This steroid is an intermediate in the biosynthesis of immunomodulatory 7-hydroxylated DHEA metabolites, and has been shown to possess thermogenic properties. The method employs polyclonal rabbit antiserum to (19E)-3beta-hydroxy-7,17,19-trione-19-O-(carboxymethyloxime):BSA conjugate and a homologous radioiodinated derivative with tyrosine methyl ester. The cross reactivity of the antiserum with structurally closest 7-hydroxyepimers of DHEA was lower than 1.7%, with DHEA 0.4%, with all other related steroid less than 0.4%. The method includes ether extraction of serum (0.5 ml), followed by RIA. Its detection limit was 0.06 pmol (18 pg)/tube, the average intra- and inter-assay coefficients of variation were 4.1% and 8.3%, respectively. Mean recovery of serum spiked with 7-oxo-DHEA varied between 78.8% and 112%. Its levels in three serum pools were compared with a low-resolution gas chromatography-mass spectrometry method with satisfactory results. The method has been used for determination of 7-oxo-DHEA in serum samples of 215 subjects (91 males and 124 females) without overt endocrine disorders, aged 5-71 years. The over-all mean+/-S.D. was 0.280+/-0.227, the median 0.239 nmol/l. No significant sex differences were recorded. The only group which differed significantly from all other ones were males below 10 years, significantly lower values than in other age groups were found also in the first two age groups of females.
Authors:
H Kazihnitková; L Zamrazilová; M Hill; O Lapcík; V Pouzar; R Hampl
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-20
Journal Detail:
Title:  Steroids     Volume:  72     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-27     Completed Date:  2007-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  342-50     Citation Subset:  IM    
Affiliation:
Institute of Endocrinology, Národní 8, 11694 Praha 1, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Animals
Antibody Specificity
Child
Child, Preschool
Dehydroepiandrosterone / analogs & derivatives*,  blood
Female
Gas Chromatography-Mass Spectrometry / methods
Humans
Male
Middle Aged
Rabbits
Radioimmunoassay / methods*
Reference Values
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
53-43-0/Dehydroepiandrosterone; 566-19-8/7-oxodehydroepiandrosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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