| A novel prokaryotic trans-2-enoyl-CoA reductase from the spirochete Treponema denticola. | |
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MedLine Citation:
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PMID: 17382934 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An NADH-dependent trans-2-enoyl-CoA reductase (EC1.1.1.36) from the Gram negative spirochete Treponema denticola was identified, expressed and biochemically characterized. The recombinant protein is a monomeric enzyme with a molecular mass of 44 kDa with a specific activity of 43+/-4.8 U/mg (micromol mg(-1)min(-1)) and K(m) value of 2.7 microM for crotonoyl-CoA. This NADH-dependent trans-2-enoyl-CoA reductase represents the first enzymatically characterized member of a prokaryotic protein family involved in a fatty acid synthesis pathway that is distinct from the familiar fatty acid synthase system. |
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Authors:
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Sara Tucci; William Martin |
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Publication Detail:
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Type: Journal Article Date: 2007-03-15 |
Journal Detail:
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Title: FEBS letters Volume: 581 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-10 Completed Date: 2007-06-13 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1561-6 Citation Subset: IM |
Affiliation:
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Institute of Botany III, Heinrich-Heine University of Duesseldorf, Universitaetsstrasse 1, 40225 Duesseldorf, Germany. sara.tucci@uni-duesseldorf.de <sara.tucci@uni-duesseldorf.de> |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AE017248 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacterial Proteins / chemistry*, genetics, isolation & purification Catalysis Cloning, Molecular Fatty Acids / biosynthesis Molecular Sequence Data Molecular Weight NADH, NADPH Oxidoreductases / chemistry*, genetics, isolation & purification Oxidoreductases Acting on CH-CH Group Donors Recombinant Proteins / chemistry*, genetics, isolation & purification Treponema denticola / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Fatty Acids; 0/Recombinant Proteins; EC 1.3.-/Oxidoreductases Acting on CH-CH Group Donors; EC 1.3.1.38/trans-2-enoyl-CoA reductase (NADPH); EC 1.6.-/NADH, NADPH Oxidoreductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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