Document Detail

A novel process related impurity and forced degradation study of synthetic wogonin: Development of a liquid chromatographic method for purity control.
MedLine Citation:
PMID:  22906689     Owner:  NLM     Status:  Publisher    
Although the chemical synthesis of wogonin (5,7-dihydroxy-8-methoxy-2-phenyl-4H-chromen-4-one) has been established, the impurity profile of this pharmaceutical agent is still not fully understood. In this study, a novel process related impurity present in the synthetic wogonin was isolated by preparative HPLC. Its structure elucidation was unambiguously carried out by NMR, HRMS and IR spectra, and it was characterized as 6-chloro-5,7-dihydroxy-8-methoxy-2-phenyl-4H-chromen-4-one (imp-1), a new compound which had never been reported before. Moreover, forced degradation studies were also carried out, and wogonin was shown to undergo isomerzation under basic stress condition to form a major degradant, 5,7-dihydroxy-6-methoxy-2-phenyl-4H-chromen-4-one (imp-2) using HPLC-Q-TOF-MS/MS technique. Finally, a selective and simple routine HPLC method was developed for simultaneous quantification of wogonin and the two related compounds. The proposed method is useful for purity control during the process development of wogonin and laboratory-prepared preparations. Their formation pathways were also discussed.
Ying Xia; Chunyong Wu; Wenyuan Liu; Feng Feng; Zhiyu Li
Related Documents :
24307599 - Extraction-free in situ derivatisation of timosaponin aiii using direct analysis in rea...
23734579 - Novel ghb-derived natural products from european mistletoe (viscum album).
19145279 - Mass spectrometric profile of the serum as a marker of experimental psychoemotional str...
18378249 - Microwave-assisted extraction of tricyclic antidepressants from human serum followed by...
22474949 - Chemical profiling of constituents of smilacis glabrae using ultra-high pressure liquid...
22573159 - Inhibitory effect of melanogenesis by 5-pentyl-2-furaldehyde isolated from clitocybe sp.
11379959 - On-line coupling of partial filling-capillary zone electrophoresis with mass spectromet...
25266169 - Authenticity and traceability of vanilla flavours by analysis of stable isotopes of car...
24986759 - Ion funnel augmented mars atmospheric pressure photoionization mass spectrometry for in...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-3
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  -     ISSN:  1873-264X     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Determination of trace human chorionic gonadotropin by using multi-wall carbon nanotubes as phosphor...
Next Document:  The effect of anionic surfactant on poliovirus particles during capillary electrophoresis.