Document Detail


A novel prespore-cell-inducing factor in Dictyostelium discoideum induces cell division of prespore cells.
MedLine Citation:
PMID:  9226449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Dictyostelium discoideum strain V12M2, at a very low cell density (approximately 10(2) cells/cm2), most amoebae differentiate into prespore cells in a salt solution containing cAMP if an adequately diluted conditioned medium (CM) is provided (Oohata, A. A. (1995) Differentiation 59, 283-288). This finding suggests the presence of factor(s) released into the medium that are involved in inducing prespore cell differentiation. In the present study, we report the presence of two types of factors that function synergistically in prespore cell induction; one is a heat-stable and dialysable factor(s) and the other is a heat-labile and non-dialysable factor termed psi (psi) factor (prespore-inducing factor). We purified and characterized the psi factor. Its relative molecular mass was determined to be 106x10(3) Mr by SDS-PAGE and 180x10(3) Mr by gel filtration HPLC, respectively. These results indicate that psi factor exists as a dimer under native conditions. In addition to inducing prespore cell differentiation, psi factor induced cell division of prespore cells in submerged culture. Our results suggest that psi factor plays important roles not only in prespore cell differentiation but also in the progress of the cell cycle in the prespore pathway in normal development.
Authors:
A A Oohata; M Nakagawa; M Tasaka; S Fujii
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  124     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-08-25     Completed Date:  1997-08-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2781-7     Citation Subset:  IM    
Affiliation:
Biological Laboratory, Kansai Medical University, Hirakata, Osaka, Japan. oohata@makino.kmu.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Cell Division
Dictyostelium / chemistry*,  cytology*
Dimerization
Fungal Proteins / physiology*
Gene Expression Regulation, Developmental
Gene Expression Regulation, Fungal
Growth Substances / physiology*
Molecular Weight
Spores, Fungal
Chemical
Reg. No./Substance:
0/Fungal Proteins; 0/Growth Substances

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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