Document Detail


A novel population of α-smooth muscle actin-positive cells activated in a rat model of stroke: an analysis of the spatio-temporal distribution in response to ischemia.
MedLine Citation:
PMID:  22805872     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a rat model of stroke, the spatio-temporal distribution of α-smooth muscle actin-positive, (αSMA+) cells was investigated in the infarcted hemisphere (ipsilateral) and compared with the contralateral hemisphere. At day 3 postischemia, αSMA+ cells were concentrated in two main loci within the ipsilateral hemisphere (Area A) in the medial corpus callosum and (Area B) midway through the striatum adjacent to the lateral ventricle. By day 7 and further by day 14, fewer αSMA+ cells remained in Areas A and B but a steady increase in the peri-infarct was observed. αSMA+ cells also expressed glial acidic fibrillary protein [GFAP: αSMA+/GFAP+ (29%); αSMA+/GFAP- (71%) phenotypes] and feline leukemia virus C receptor 2 (FLVCR2), but not ED1(microglia) and established markers of pericytes normally located in vascular wall. αSMA+ cells were also located close to the subventricular zones (SVZ) adjacent to Areas A and B. In conclusion, αSMA+ cells have been identified in a spatial and temporal sequence from the SVZ, at intermediate loci and in the vicinity of the peri-infarct. It is hypothesized that novel populations of αSMA+ precursors of pericytes are born on the SVZ, migrate into the peri-infarct region and are incorporated into new vessels of the peri-infarct regions.
Authors:
Varun Sharma; Tina W Ling; Sarah S Rewell; David L Hare; David W Howells; Angela Kourakis; Peter J Wookey
Publication Detail:
Type:  Journal Article     Date:  2012-07-18
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  32     ISSN:  1559-7016     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-06     Completed Date:  2013-01-11     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2055-65     Citation Subset:  IM    
Affiliation:
Cardiology Department, Austin Health, Melbourne, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
Biological Markers
Brain Ischemia / pathology*
Ectodysplasins / metabolism
Fluorescent Antibody Technique
Functional Laterality / physiology
Immunohistochemistry
Infarction, Middle Cerebral Artery / pathology
Ki-67 Antigen / metabolism
Macrophages / metabolism
Male
Microglia / metabolism
Microscopy, Confocal
Myocytes, Smooth Muscle / metabolism*,  pathology*,  ultrastructure
Paraffin Embedding
Rats
Rats, Inbred SHR
Stroke / pathology*
Chemical
Reg. No./Substance:
0/Actins; 0/Biological Markers; 0/Ectodysplasins; 0/Ki-67 Antigen
Comments/Corrections

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