| The novel plasminogen receptor, Plg-RKT, regulates macrophage migration. | |
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MedLine Citation:
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PMID: 21940822 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Localization of plasmin on macrophages and activation of pro-MMP-9 play key roles in macrophage recruitment in the inflammatory response. These functions are promoted by plasminogen receptors exposing C-terminal basic residues on the macrophage surface. Recently, we identified a novel transmembrane plasminogen receptor, Plg-R(KT), which exposes a C-terminal lysine on the cell surface. Here we investigated the role of Plg-R(KT) in macrophage invasion, chemotactic migration and recruitment. Plg-R(KT) was prominently expressed in membranes of human peripheral blood monocytes and monocytoid cells. Plasminogen activation by uPA was markedly inhibited (by 39 %) by treatment with anti-Plg-R(KT) mAb. Treatment of monocytes with anti-Plg-R(KT) mAb substantially inhibited invasion through the representative matrix, Matrigel, in response to MCP-1 (by 54 % compared to isotype control). Furthermore, chemotactic migration was also inhibited by treatment with anti-Plg-R(KT) mAb (by 64%). In a mouse model of thioglycollate-induced peritonitis, anti-Plg-R(KT) mAb markedly inhibited macrophage recruitment (by 58%), concomitant with a reduction in pro-MMP-9 activation in the inflamed peritoneum. Treatment with anti-Plg-R(KT) mAb did not further reduce the low level of macrophage recruitment in plasminogen null mice. Thus, Plg-R(KT) plays a key role in plasminogen-dependent regulation of macrophage invasion, chemotactic migration and recruitment in the inflammatory response. |
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Authors:
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Shahrzad Lighvani; Nagyung Baik; Jenna E Diggs; Sophia Khaldoyanidi; Robert J Parmer; Lindsey A Miles |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-22 |
Journal Detail:
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Title: Blood Volume: - ISSN: 1528-0020 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Cell Biology, The Scripps Research Institute, La Jolla, CA, United States; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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