Document Detail


A novel piscine vitellogenin gene: structural and functional analyses of estrogen-inducible promoter.
MedLine Citation:
PMID:  10022768     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Oreochromis aureus vitellogenin, OaVtg, gene spans 9 kb and contains 34 exons. Its transcription start site is located 15 bp upstream of the translational start codon. Although the OaVtg promoter has a nonconsensus TATA, transient transfection assay showed that this promoter is capable of driving basal transcription. Two imperfect estrogen response elements: EREp (proximal) and EREd (distal) are located in the promoter at - 532 and - 1352, respectively. In competition gel mobility-shift assays, only EREp exhibited specific binding of the recombinant estrogen receptor protein, GST-C/D OaER. Another imperfect ERE (EREexon2) was detected within exon 2 of the OaVtg gene. This is a novel finding for a vitellogenin (Vtg) gene. EREexon2 similarly showed specific recognition of GST-C/D OaER. Both EREp and EREexon2 showed comparable binding affinities as consensus ERE. In transient transfections, the OaVtg promoter, EREp and EREd elicited significant increase in estrogen-dependent synthesis of CAT protein. Hence, we propose that the non-consensus OaVtg EREs contribute to the estrogen-dependent regulation of the OaVtg gene in vivo.
Authors:
B Y Teo; N S Tan; E H Lim; T J Lam; J L Ding
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  146     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-05-03     Completed Date:  1999-05-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  103-20     Citation Subset:  IM    
Affiliation:
National University of Singapore, Department of Biological Sciences, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Chloramphenicol O-Acetyltransferase / genetics
Escherichia coli / genetics
Estrogens / pharmacology*
Exons
Female
Gene Expression
Introns
Molecular Sequence Data
Promoter Regions, Genetic*
Receptors, Estrogen / genetics,  metabolism
Recombinant Proteins / metabolism
Regulatory Sequences, Nucleic Acid
Response Elements
Tilapia / genetics*
Transcription, Genetic
Vitellogenins / chemistry,  genetics*
Chemical
Reg. No./Substance:
0/Estrogens; 0/Receptors, Estrogen; 0/Recombinant Proteins; 0/Vitellogenins; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase

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