Document Detail


A novel nucleolar protein, PAPA-1, induces growth arrest as a result of cell cycle arrest at the G1 phase.
MedLine Citation:
PMID:  15556297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have identified a novel nucleolar protein, PAP-1-associated protein-1 (PAPA-1), after screening the interacting proteins with Pim-1-associated protein-1 (PAP-1), a protein that is a phosphorylation target of Pim-1 kinase. PAPA-1 comprises 345 amino acids with a basic amino-acid cluster. PAPA-1 was found to be localized in the nucleolus in transfected HeLa cells, and the lysine/histidine cluster was essential for nucleolar localization of PAPA-1. PAPA-1 protein and mRNA expression decreased upon serum restimulation of starvation-synchronized cells, which displayed maximum level of PAPA-1 expression at G0 and early G1 phase of the cell cycle. Ectopic expression of PAPA-1 induced growth suppression of cells, and the effect was dependent on its nucleolar localization in established HeLa cell lines that inducibly express PAPA-1 or its deletion mutant under the control of a tetracycline-inducible promoter. Furthermore, when PAPA-1-inducible HeLa cells were synchronized by thymidine, colcemid or mimosine, and then PAPA-1 was expressed, the proportion of cells at the G1 phase was obviously increased. These results suggest that PAPA-1 induces growth and cell cycle arrests at the G1 phase of the cell cycle.
Authors:
Taruho S Kuroda; Hiroshi Maita; Takanori Tabata; Takahiro Taira; Hirotake Kitaura; Hiroyoshi Ariga; Sanae M M Iguchi-Ariga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  340     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-11-23     Completed Date:  2004-12-28     Revised Date:  2008-09-11    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  83-98     Citation Subset:  IM    
Affiliation:
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita, Sapporo 060-0812, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB054537;  AB054538
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
BALB 3T3 Cells
Base Sequence
Blotting, Northern
Carrier Proteins / genetics*,  metabolism,  physiology
Cell Cycle / physiology*
Cell Cycle Proteins
Cell Division / physiology
Cell Line
Cell Nucleolus / metabolism
Cell Nucleus / metabolism
Cloning, Molecular
DNA, Complementary / chemistry,  genetics
G1 Phase / physiology*
Gene Expression Profiling
Hela Cells
Humans
L Cells (Cell Line)
Male
Mice
Molecular Sequence Data
Mutation
NIH 3T3 Cells
Nuclear Localization Signals / genetics
Nuclear Proteins / genetics*,  metabolism,  physiology
Protein Binding
RNA, Messenger / genetics,  metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Transfection
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Cell Cycle Proteins; 0/DNA, Complementary; 0/INO80B protein, human; 0/Nuclear Localization Signals; 0/Nuclear Proteins; 0/PAPA-1 protein, mouse; 0/RNA, Messenger

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