Document Detail


A novel nuclear substructure, ND10: distribution in normal and neoplastic human tissues.
MedLine Citation:
PMID:  9852287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ND10 are recently characterized nuclear domains that are composed of 0.5 microm sized, precisely circumscribed dots in cultured human cell lines. To investigate the distribution and number of ND10 on various types of normal and neoplastic human tissues, we carried out immunostaining and immunoprecipitation analyses with monoclonal antibodies 138 and 1150. The number of ND10 varied from 1 to 10 or more in various tissues as did their size. ND10 were diffusely located in early embryonic and normal tissues, except for the exocrine and endocrine cells of the pancreas and for hepatocytes. In normal squamous mucosa, basal cells had more ND10 than did differentiated superficial squamous cells. The number and size of ND10 were markedly increased in malignant neoplasms but were similar in benign tumors and corresponding normal tissues. Sex hormone-related normal tissues, such as the endometrium or myometrium, and neoplasms strongly stained for ND10. The distribution pattern of ND10 in human tissues indicates that they are conserved nuclear substructures that are closely associated with cellular differentiation, hormonal stimulation, and oncogenesis.
Authors:
Y Cho; I Lee; G G Maul; E Yu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  1     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1999-03-01     Completed Date:  1999-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  717-24     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Ulsan College of Medicine, Songpa-gu, Seoul 138-736, Korea.
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MeSH Terms
Descriptor/Qualifier:
Digestive System / cytology
Endocrine Glands / cytology
Female
Genitalia, Female / cytology
Genitalia, Male / cytology
Humans
Male
Microscopy, Fluorescence
Neoplasms / chemistry*,  metabolism,  pathology
Nervous System / cytology
Nuclear Matrix / chemistry*,  metabolism,  pathology
Nuclear Proteins / analysis*,  metabolism
Reference Values
Respiratory System / cytology
Tumor Cells, Cultured / chemistry,  metabolism,  pathology
Urinary Tract / cytology
Chemical
Reg. No./Substance:
0/Nuclear Proteins

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