| A novel, noninvasive method for the measurement of intestinal fat absorption. | |
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MedLine Citation:
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PMID: 15236180 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND & AIMS: The goal of the study was to facilitate fat balance measurements with an appropriate intestinal marker for the transit of dietary fat and thereby eliminate the need for complete diet and fecal collections. METHODS: Dietary fat containing 5% sucrose polybehenate was fed in a semisynthetic diet to rats and mice. Fat absorption was calculated from the ratios of behenic acid to other fatty acids in diet and feces as analyzed by gas chromatography of fatty acid methyl esters. The method was validated by measuring absorption of well-absorbed (safflower oil) and poorly absorbed (olestra; calcium soaps) dietary fats. The animals were fed meals containing test fats for 2 or 3 days, and fecal samples were collected. Fecal samples of approximately 10 mg (single fecal pellet from mice) were assayed. RESULTS: The method yielded values that were consistent with complete absorption of safflower oil and the nonabsorbability of olestra and calcium soaps. The results were reproducible and consistent among individual fecal aliquots. The method was compared with traditional fat-balance methods in animals fed both high- and low-fat diets. CONCLUSIONS: Sucrose polybehenate is an appropriate marker that allows the rapid measurement of fat absorption by analyzing aliquots of <1% of total feces. The method is noninvasive, does not require isotope analyses, and can be carried out as part of an animal's normal feeding regimen. The method may be a facile technique to assess fat absorption measurements in humans. |
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Authors:
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Ronald J Jandacek; James E Heubi; Patrick Tso |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Gastroenterology Volume: 127 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-07-05 Completed Date: 2004-09-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
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Languages: eng Pagination: 139-44 Citation Subset: AIM; IM |
Affiliation:
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University of Cincinnati, College of Medicine, Department of Pathology, Ohio 45267, USA. ronald.jandacek@uc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Diagnostic Techniques, Digestive System Dietary Fats / metabolism* Fatty Acids / pharmacokinetics* Feces / chemistry Female Intestinal Absorption / drug effects, physiology* Male Mice Mice, Inbred C57BL Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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DK59630/DK/NIDDK NIH HHS; R01 DK56910/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; 0/Fatty Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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