| A novel nitrite biosensor based on the direct electron transfer of hemoglobin immobilized on CdS hollow nanospheres. | |
| | |
MedLine Citation:
|
PMID: 18424126 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A novel nitrite biosensor based on the direct electron transfer of hemoglobin (Hb) immobilized on CdS hollow nanospheres (HS-CdS) modified glassy carbon electrode was constructed. The direct electron transfer of Hb showed a pair of redox peaks with a formal potential of -286 mV (vs. SCE) in 0.1M pH 7.0 phosphate buffer solution. It was a surface-controlled electrode process involving a single proton transfer coupled with a reversible one-electron transfer for each heme group of Hb. HS-CdS had a large specific surface area and good biocompatibility and had a better electrochemical response than that of solid spherical CdS. The immobilized Hb on HS-CdS displayed an excellent response to NO(2)(-) with one irreversible electrode process for NO reduction. Under optimal conditions, the biosensor could be used for the determination of NO(2)(-) with a linear range from 0.3 to 182 microM and a detection limit of 0.08 microM at 3 sigma based on the irreversible reduction of NO. HS-CdS provided a good matrix for protein immobilization and had a promising application in constructing sensors. |
| | |
Authors:
|
Zhihui Dai; Hongyan Bai; Mei Hong; Yinyan Zhu; Jianchun Bao; Jian Shen |
Related Documents
:
|
19071736 - Direct electrochemistry and electroanalysis of hemoglobin adsorbed in self-assembled fi... 21137956 - A kind of synthetic nanopillar arrays for studying single biomolecule. 16852366 - X-ray absorption spectroscopic study on the electronic structure of li(1)(-)(x)()copo(4... 21466166 - Scanning photoionization microscopy: ultrafast plasmon-mediated electron ejection dynam... 16669456 - Antifungal effects of a tissue conditioner coating agent with tio2 photocatalyst. 22690866 - Spin-coupled theory for 'n electrons in m orbitals' active spaces. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-14 |
Journal Detail:
|
Title: Biosensors & bioelectronics Volume: 23 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 2008 Jul |
Date Detail:
|
Created Date: 2008-05-19 Completed Date: 2008-08-19 Revised Date: 2009-07-14 |
Medline Journal Info:
|
Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
|
Languages: eng Pagination: 1869-73 Citation Subset: IM |
Affiliation:
|
Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, PR China. daizhihuii@njnu.edu.cn |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adsorption Biosensing Techniques / instrumentation* Cadmium Compounds / chemistry* Electrochemistry / instrumentation* Equipment Design Equipment Failure Analysis Hemoglobins / chemistry* Nanospheres / chemistry*, ultrastructure Nitrites / analysis*, chemistry Porosity Protein Binding Selenium Compounds / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Cadmium Compounds; 0/Hemoglobins; 0/Nitrites; 0/Selenium Compounds; 1306-24-7/cadmium selenide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Real-time optical detection of methicillin-resistant Staphylococcus aureus using lytic phage probes.
Next Document: Towards beyond-1 GHz solution NMR: internal 2H lock operation in an external current mode.