Document Detail

A novel nitrite biosensor based on the direct electron transfer of hemoglobin immobilized on CdS hollow nanospheres.
MedLine Citation:
PMID:  18424126     Owner:  NLM     Status:  MEDLINE    
A novel nitrite biosensor based on the direct electron transfer of hemoglobin (Hb) immobilized on CdS hollow nanospheres (HS-CdS) modified glassy carbon electrode was constructed. The direct electron transfer of Hb showed a pair of redox peaks with a formal potential of -286 mV (vs. SCE) in 0.1M pH 7.0 phosphate buffer solution. It was a surface-controlled electrode process involving a single proton transfer coupled with a reversible one-electron transfer for each heme group of Hb. HS-CdS had a large specific surface area and good biocompatibility and had a better electrochemical response than that of solid spherical CdS. The immobilized Hb on HS-CdS displayed an excellent response to NO(2)(-) with one irreversible electrode process for NO reduction. Under optimal conditions, the biosensor could be used for the determination of NO(2)(-) with a linear range from 0.3 to 182 microM and a detection limit of 0.08 microM at 3 sigma based on the irreversible reduction of NO. HS-CdS provided a good matrix for protein immobilization and had a promising application in constructing sensors.
Zhihui Dai; Hongyan Bai; Mei Hong; Yinyan Zhu; Jianchun Bao; Jian Shen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-14
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  23     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-08-19     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1869-73     Citation Subset:  IM    
Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, PR China.
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MeSH Terms
Biosensing Techniques / instrumentation*
Cadmium Compounds / chemistry*
Electrochemistry / instrumentation*
Equipment Design
Equipment Failure Analysis
Hemoglobins / chemistry*
Nanospheres / chemistry*,  ultrastructure
Nitrites / analysis*,  chemistry
Protein Binding
Selenium Compounds / chemistry*
Reg. No./Substance:
0/Cadmium Compounds; 0/Hemoglobins; 0/Nitrites; 0/Selenium Compounds; 1306-24-7/cadmium selenide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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