Document Detail


A novel myogenic cell line with phenotypic properties of muscle progenitors.
MedLine Citation:
PMID:  17957349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Skeletal myogenesis is a multistep process starting with progenitor cell proliferation, followed by their exit from the cell cycle, differentiation, alignment, and fusion to form multinucleated myotubes, typical of the differentiated muscle tissue. While the molecular players involved in early myogenesis have been extensively characterized, information about the later steps of the process is scanty. Here, we describe a novel myogenic cell line (MYOP7), composed of highly proliferating Sca-1+ muscle precursor cells, which can be induced to terminally differentiate into spontaneously contracting multinucleated myotubes. By performing high-density microarray analysis on these cells, we identified a series of genes, differentially expressed in proliferating vs differentiating conditions, which are candidates to play a major role in the later phase of myogenesis. In addition, we confirmed that the late stages of muscle differentiation are characterized by a marked upregulation of the cellular receptors for the vascular endothelial growth factor.
Authors:
Serena Zacchigna; Even K Østli; Nikola Arsic; Lucia Pattarini; Mauro Giacca; Srdjan Djurovic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-24
Journal Detail:
Title:  Journal of molecular medicine (Berlin, Germany)     Volume:  86     ISSN:  1432-1440     ISO Abbreviation:  J. Mol. Med.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-11     Completed Date:  2009-05-11     Revised Date:  2011-07-08    
Medline Journal Info:
Nlm Unique ID:  9504370     Medline TA:  J Mol Med (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  105-15     Citation Subset:  IM    
Affiliation:
Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Cell Line*
Mice
Muscle Development*
Muscle, Skeletal / cytology*,  physiology
Receptors, Cell Surface / genetics
Stem Cells / cytology
Up-Regulation
Vascular Endothelial Growth Factor A / genetics*
Chemical
Reg. No./Substance:
0/Receptors, Cell Surface; 0/Vascular Endothelial Growth Factor A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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