Document Detail


A novel mutation lacking the bromodomain of the transcriptional coactivator p300 in the SiHa cervical carcinoma cell line.
MedLine Citation:
PMID:  11181085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transcriptional coactivator p300, a histone acetyltransferase (HAT), plays key roles in the regulation of cell proliferation and differentiation. p300 is targeted by viral oncoproteins, and mutations of p300, accompanied by inactivation of the second allele, have been reported in certain types of cancers originating in the epithelium. Here, we identified a homozygous p300 deletion of exons 15--18 in the SiHa cervical carcinoma cell line, which results in an in-frame deletion that causes specific loss of the bromodomain, a conserved domain implicated in the regulation of HAT activity. Furthermore, we show that the mutation severely impaired its ability to activate the p21(WAF1/CIP1) promoter in transient reporter assay. These results suggest a critical role for the bromodomain in p300 functions as a tumor-suppressor gene.
Authors:
T Ohshima; T Suganuma; M Ikeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  281     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  569-75     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
Department of Oral Microbiology, Tsurumi University, School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Binding Sites / genetics
Blotting, Western
Cell Line
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / genetics
DNA, Complementary / genetics
Female
Gene Expression Regulation, Neoplastic
Hela Cells
Humans
K562 Cells
Luciferases / genetics,  metabolism
Mutation*
Nuclear Proteins / chemistry,  genetics*,  metabolism
Promoter Regions, Genetic / genetics
Protein Structure, Tertiary
RNA, Messenger / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Deletion
Trans-Activators / chemistry,  genetics*,  metabolism
Tumor Cells, Cultured
U937 Cells
Uterine Cervical Neoplasms / genetics*,  metabolism,  pathology
Chemical
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/DNA, Complementary; 0/Nuclear Proteins; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; 0/Trans-Activators; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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